Assessment of cardiovascular risk factors in people with HIV infection treated with ART in rural South Africa: a cross sectional study
© Mashinya et al. 2015
Received: 5 September 2015
Accepted: 26 November 2015
Published: 10 December 2015
The risk of cardiovascular diseases (CVD) in human immunodeficiency virus (HIV) infected people on antiretroviral therapy (ART) from some rural parts of Africa is not well known. We assessed CVD risk factors, the estimated 5-year Data collection on adverse effects of anti-HIV drugs (DA.) risk score and the 10-year Framingham risk score in persons with HIV infection on ART in a rural area in South Africa.
A cross-sectional study in which the data on demographic, lifestyle, and chronic disease were collected using the World Health Organization Stepwise approach to surveillance questionnaire. Biochemical parameters were tested using standard biochemical methods. CD4 counts were performed using PIMA analyser and viral load was tested using the branched deoxyribonucleic acid technique. Student t test and Chi square test were used on continuous and categorical variables respectively. Bivariate and multivariate logistic regression were used to analyze predictors of CVD risk factors. Estimates of 5 and 10-year CVD risk were calculated using online tools. The Cohen’s kappa coefficient was used to assess the agreement between CVD risk equations.
The mean age of participants was 44.8 ± 11.8 years; 79.9 % were females. Most of the participants (85 %) had an undetectable viral load and a mean CD4 count of 462 ± 235 cell/mm3. The most common CVD risk factors were low high density lipoprotein cholesterol (HDL-C) (43.8 %), hypercholesterolaemia (33.2 %) and a high Apolipoprotein (Apo) B/ApoA ratio (45.4 %).Using the Framingham equation, 6.7 % of participants had a moderate to high 10-year CVD risk while the DAD risk equation showed that 31.1 % of participants had a moderate to high 5-year CVD risk. Most participants had a low CVD risk by both risk equations. The level of agreement between the two risk equations was 73.8 % (k = 0.23; 95 % CI 0.10–0.35; p value 0.001).
CVD risk factors were common among this rural population on ART. The high proportion of participants with a moderate to high CVD risk, observed with the DAD risk equation, clearly represents a considerable health burden that can possibly be reduced by increasing educational programs on CVD prevention for people on ART. There is however a need to develop and evaluate a race/ethnicity-specific CVD risk estimation tool for HIV infected Africans.
KeywordsCardiovascular disease risk Human immunodeficiency virus Antiretroviral therapy
Human immunodeficiency virus (HIV) or antiretroviral therapy (ART) through direct or indirect mechanisms may induce diabetes mellitus, dyslipidaemia, hypertension, lipodystrophy and endothelial dysfunction . Furthermore, some studies have shown that traditional risk factors for cardiovascular diseases (CVD) such as low physical activity, increased body mass index (BMI), smoking, are frequently present in HIV infected people [2, 3].
A limited number of studies have assessed CVD risk factors in HIV infected people on ART from low-income countries [4–6]. Furthermore, the number of CVD risk factors, type and duration of ART reported in these studies varied. Most studies on CVD risk factors in persons with HIV infection were done in Western countries [7–11]. While some of these studies were conducted in areas with people of a low-socio-economic class similar to developing countries, differences in ethnicity, environmental, genetic factors, [12–14] and populations’ uptake of smoking cessation campaign , may have influenced prevalence rates of CVD risk factors.
Studies on CVD risk factors among HIV infected people are however scarce in South Africa [15, 16]. A recent attempt to describe the CVD risk in rural people on ART from Mpumalanga in South Africa was made , but the use of ART was self-reported and according to the authors, the prevalence of CVD risk factors in that rural population on ART may be underestimated since there remains considerable stigma associated with HIV. In South Africa, the intersection of an epidemiological transition , a high number of people living with HIV  and a widespread adult treatment coverage that was close to 80 % by mid-2011 , presents a high risk of CVD among people infected with HIV and needs to be investigated. While CVDs are preventable, little is known regarding CVD risk factors in HIV infected rural South Africans on ART . We determined the prevalence of CVD risk factors, the estimated 5-year DAD risk score and the 10-year Framingham risk score in persons with HIV infection on ART in a rural area in South Africa.
A cross-sectional study was conducted in the primary health care clinics, Seobi-Dikgale, Sebayeng and Dikgale. At least 74 people with HIV infection are treated at Seobi-Dikgale clinic, while 373 people and 377 people with HIV infection are treated at Sebayeng and Dikgale clinics respectively. The three clinics are situated within the Dikgale Health and Demographic Surveillance System (HDSS) site. Dikgale HDSS site is situated about 70 km to the Northeast of Polokwane, the capital city of Limpopo Province. The site consists of 15 villages. Dwellings in Dikgale HDSS are a mix of shacks, conventional brick houses and traditional mud huts in the Limpopo province of South Africa.
During one month all patients, at least 15 years old who came to collect their ART were asked to participate in the study. Those willing to participate, were advised on the participation dates and time (7:00am) at their clinic and were advised to provide a fasting sample. Participants who could not come on the scheduled date were visited in their homes on an arranged date and participated. Participants were only included after written informed consent was obtained through the completion of a consent form approved by the Medical University of South Africa (MEDUNSA) Ethics committee. In the case of minors, written informed consent was obtained from their legal guardians. Pregnant women were excluded.
Ethical approval was obtained from the Ethics Committee of University of Limpopo, Medunsa campus. Permission to conduct the study in the Dikgale HDSS site clinics was obtained from the Department of Health-Provincial office and Primary Health Care Capricorn District office.
The study was performed from September 2013 to March 2014. The World Health Organization stepwise approach to surveillance (WHO STEPS) questionnaire  was used to obtain information on dietary intake, physical activity, socio-demographic, tobacco use, alcohol consumption and medical history.
Anthropometric and blood pressure measurements
Anthropometric and blood pressure measurements were taken following procedures as previously described . In brief, weight was measured to the nearest 0.1 kg, using Omron BF 400 (Omron Healthcare, Japan). Height was measure to the nearest 0.1 cm, using a stadiometer. BMI was calculated as weight (kg)/height (m2). A BMI was considered as normal (18.50–24.99), overweight (25.00–29.99) and obese (≥30.00) . Waist circumference and hip circumference were measured to the nearest 0.1 cm, using a measuring tape. Both parameters were used to calculate the waist to hip ratio. The Omron M5-1 (Omron Healthcare, Kyoto, Japan) was used to measure blood pressure. High blood pressure was defined as a systolic blood pressure (SBP) above 140 mmHg and/or a diastolic blood pressure (DBP) above 90 mmHg  and/or self-reported history of antihypertensive medication. Metabolic syndrome was defined as any three of the following five risk factors; abdominal obesity (waist circumference → 88 cm for females and >102 cm for males), high TG concentration (≥1.7 mmol/l), low HDL-C concentration (≤1.3 for females and ≤1.1 for males), high Blood pressure and raised fasting plasma glucose concentration (>7 mmol/l) .
Fasting venous blood samples were drawn by registered nurses. Whole blood was used to measure CD4 counts on the day of collection. Serum from clotted blood and plasma from whole blood were separated through centrifugation at 2000 rpm for 15 min. Glucose was analysed soon after centrifugation using plasma from sodium fluoride tubes. The remaining samples were stored at −80 °C until analysis.
Triglycerides (TGs), total cholesterol (TC), high density lipoprotein (HDL) cholesterol and glucose levels were determined on ILab 300 Plus Chemistry System (Instrumentation Laboratory Company, Milan, Italy). Apolipoprotein B (ApoB), Apolipoprotein A1 (ApoA1) were measured on the IMMAGE Immunochemistry System (Beckman Coulter, USA). CD4 count was measured using the Pima Analyser (Inverness Medical, Tokyo, Japan). Viral load testing, using the branched deoxyribonucleic acid (DNA) technique (Siemens, South Africa) was performed by Toga Molecular Biology and Pathology medical laboratory that is South African National Accreditation System (SANAS) accredited for ISO 17025.
Statistical analysis was performed using Statistical Package for Social Science version 22. Frequency histogram and line graph was used to check normality of continuous variable. The independent Student t-test was used to compare continuous variables and the Chi square test was used to compare categorical variables between males and females. Factors significant at p-value less or equal to 0.25 in bivariate regression were considered as candidates for multivariate modelling. Multivariate logistic regression was used to determine the significant predictors of CVD risk factors. The level of significance for statistical analysis was set at less than 0.05. Framingham 10-year CVD risk estimation was calculated for each participant above 20 years, with no diabetes and no history of CVD, by entering the following variables: age, gender, TC, HDL-C, SBP, smoking status and current treatment for high blood pressure, as required by the Framingham risk model tool . Participants were regarded as low risk, moderate risk, or high risk when the risk score for developing CVD in 10 years was <10, 10–20 or >20 % respectively . Variables included in the 5-year DAD risk estimation tool were age, sex, SBP, TC, HDL-C, diabetes mellitus, smoking status, family history of CVD, current use of abacavir, indinavir, or lopinavir and duration on indinavir and lopinavir . The risk of developing coronary heart disease in the next 5-years was regarded as low (<1 %), moderate (1–5 %), high (5–10 %), or very high (>10 %) . The level of agreement between DAD and Framingham risk equations was determined using Cohen’s Kappa coefficient with 95 % CI. For comparison with Framingham, participants with high and very high scores according to the DAD equation were combined and considered as high risk group. Kappa coefficients was interpreted as poor agreement (<0), slight agreement (0.0–0.20), fair agreement (0.21–0.40), moderate agreement (0.41–0.60), substantial agreement (0.61–0.80) and perfect agreement (0.81–1.00) .
Characteristics of participants
Two hundred and fourteen HIV infected people on ART, participated in the study. Of which 171 (79.9 %) were females and 43 (20.1 %) were males. The mean age of ART participants was 44.8 ± 11.8 years and males were significantly older than females (49.9 ± 11.1 vs 43.5 ± 11.6, p = 0.001). The percentage of unmarried participants was 164 (76.6 %) with more females than males (80.1 vs 62.8 %, p < 0.05). About 45.8 % had secondary level of education. Unemployment was 69.6 %, and higher in females (75.4 %) than in males (46.5 %).
The majority of participants were on efavirenz (86 %) and nevirapine (12.5 %) based ART. Only three of the participants (1.5 %) were on a lopinavir/ritonavir based ART regimen. The mean duration of ART in this study was 36 months (range 1–121 months). Most of the participants (85 %) had an undetectable viral load and a mean CD4 count of 462 ± 235 cell/mm3.
Prevalence of behavioural and metabolic CVD risk factors
Demographics, HIV information and CVD risk factors in ART treated HIV infected participants by gender
All participants N = 214, n (%)
Females N = 171, n (%)
Males N = 43, n (%)
44.8 ± 11.8
43.5 ± 11.6
49.9 ± 11.1
CD4 count (cells/mm3)
461.9 ± 235.3
485.5 ± 234.1
364.3 ± 216.6
Viral load (copies/ml)
Mean duration of ART in months (N = 200)
36.1 ± 24.4
37.0 ± 24.3
32.5 ± 24.6
NVP (NNRTI)-based ART*
EFV (NNRTI)-based ART
Alluvia (PI)-based ART
CVD risk factors
29 (17 %)
18 (41.9 %)
Physical activity <600 MET-min/wk)
37 (29.1 %)
1 (3.3 %)
Fruit and vegetables intake (<5 servings/day)
High TG/HDL-C (≥1.49)
Predictors of metabolic CVD risk factors
Predictors of Metabolic CVD risk factors among ARV treated HIV infected people
Metabolic CVD risk factors
High TC/ HDL-C
High Apo B/Apo A
4.67 1 (1.94–11.2)
5.66 1 (1.36–23.5)
2.27 1 (1.04–4.95)
2.91 1 (1.12–7.55)
3.29 1 (1.35–8.01)
1.10 1 (1.02–1.18)
2.94 1 (1.13–7.65)
0.08 1 (0.01–0.63)
3.82 1 (1.51–9.63)
3.83 1 (1.73–8.46)
0.29 1 (0.09–0.90)
0.31 1 (0.11–0.89)
2.10 1 (1.09–4.05)
CD 4 count
Abd. obesity by PA
Gender by ARV months
Framingham risk scores
None of the 164 participants according to the Framingham estimation had a high risk of developing a CVD event in the next 10 years. However, about 6.7 % had a moderate risk and majority had a low risk (93.3 %).
DAD risk scores
Of the 164 participants, 68.9 % had a low risk, 27.4 % had a moderate risk and 3.7 % had a combined high and very high risk for developing a CVD event in next 5-years. However, considering all (214) participants, 66.8 % had a low risk, 29 % had a moderate risk, 1.9 % had a high risk and 2.3 % had a very high risk for developing CVD in next 5-years.
Comparison between CVD risk estimation using DAD and Framingham equations
Cardiovascular disease risk
FRAMINGHAM (N = 164)
Low risk (<10 %)
Moderate risk (10–20 %)
High risk (>20 %)
DAD (N = 164)
Low risk (<10 %)
Moderate risk (1–5 %)
High risk and very high risk (>5 %)
95 % CI for Kappa
DAD risk estimations on all participants
Low risk (<1 %)
Moderate risk (1–5 %)
High risk (5.1–10 %)
Very high risk (>10 %)
All participants (N = 214)
143 (66.8 %)
62 (29 %)
4 (1.9 %)
5 (2.3 %)
Although our study population was young, using the DAD risk equation 31.1 % of participants had a 5-year moderate to high CVD risk. Several factors may play a role in causing this high risk. In our study, most people were physically active (75.8 %). However, the use of a different instrument for data collection may explain variations observed in physical activity between our study and others [5, 31]. Most participants in our study cited non availability of fruits and vegetables coupled with unaffordability as reasons for low intake. This low intake remains a major challenge as it increases the risk of nutritional deficiencies [32, 33] and CVD incidence and mortality . Consistent with our findings, a low intake of fruit and vegetable was reported among the general population of Dikgale HDSS .
A high rate of unemployment was observed in this study, as was reported in the general population in the same community . This high unemployment rate coupled with stigma related to HIV infection, may predispose the HIV infected people to high levels of stress. Given that chronic stress predicts the occurance of CVD , interventions aimed at creation of jobs among HIV infected people may therefore play an important role in indirectly reducing the risk for CVD, by alleviating stress associated with unemployment.
Among metabolic risk factors, hypertension was observed in nearly a quarter of participants, consistent with a study in Senegal . However other studies that included younger populations reported lower prevalence rates [6, 38–40]. The prevalence of hypertension was similar between males and females. Of the 42 HIV infected participants with a history of hypertension and were on medication, approximately 60 % had their blood pressure controlled, while 40 % had a raised blood pressure despite being on medication. Factors contributing to the uncontrolled blood pressure include poor adherence to treatment, high salt intake and large alcohol consumption (>3 drinks per day) . Older age was a predictor of hypertension, and people with a low viral load were more likely to be hypertensive. Diabetes mellitus (DM) in males and females was low as reported among people on ART in other African studies [5, 38, 42, 43]. Unlike our study findings, a high prevalence of diabetes mellitus was reported among HIV negative and ART naïve HIV positive people in Dikgle HDSS , probably due to an older age than that of our cohort. The overall prevalence of diabetes mellitus among South Africans aged 30 years and above is estimated at 9 % equaling a 9.3 % prevalence for United States of America . South Africa is undergoing epidemiological transition and prevalence of diabetes mellitus is thus expected to rise in future . The prevalence of obesity (10 %) and abdominal obesity (22 %) in our study differed from prevalences reported from Africa [6, 46] and Asia [40, 47]. According to Crum-Ciaflone et al. (2008) , the variations in obesity and abdominal obesity may partly be explained by differences in ART duration and the different cut-off values for the BMI and waist circumference used in various studies. Females in our study were more likely to be obese than males, although the difference was not significant due to the small number of participants . The prevalence of metabolic syndrome was low and similar in males and females. Reported prevalences of metabolic syndrome vary, possibly resulting from its heterogeneous nature and variations in prevalence of its components [39, 50–52].
The prevalence of hypertriglyceridaemia was low, maybe due to the NNRTI based regimen used. The use of a stavudine containing regimen in certain other studies [40, 53, 54] may explain why a prevalence more than twice as high was observed in those studies. In our study high concentrations of TG, were more common in males than in females, but gender could not predict a high TG concentration in multivariate analysis. Abdominal obesity and older age were significant predictors of hypertriglyceridaemia as previously reported . High TG levels in our study were associated with low intake of fruit and vegetables as has been reported in other studies [32, 33]. Although ART increases lipid levels, HDL-C may not return to normal levels thus high prevalence of low HDL-C has been observed in this and other studies [6, 38, 39]. Older age and high viral load were independent predictors of a low HDL-C concentration. These results show the importance of suppressing the viral load to minimize the risk for developing low HDL-C concentration when people on ART become older. Hypercholesterolaemia was present in a third of the participants which is similar to results from other studies [5, 6, 38, 39]. Visceral lipohypertrophy increased the likelihood of having a high total cholesterol concentration. None of our participants was using lipid lowering drugs, possibly accounting for the high proportions of dyslipidaemia. Lipid ratios are regarded as better predictors of CVD than individual lipids [56, 57]. A high ApoB/ApoA ratio was present in nearly 50 % of participants a possible reflection of high prevalence of low HDL-C. Its association with high viral load in our study predicts the benefits of effective suppression of virus load. Nearly 15 % of participants mostly males had a high TC/HDL-C ratio. Older age, longer ART duration and male gender, were predictors of a high TC/HDL-C ratio. These findings have important implications as these variables may increase the risk of developing CVD. A high TG/HDL-C ratio present in nearly a fifth of the participants mostly males was associated with abdominal obesity.
An earlier study conducted among ART naïve HIV infected and HIV negative people in Dikgale Health and Demographic Surveillance System site reported a high prevalence of CVD risk factors which was similar between the two groups , but a worse CVD risk profile compared to that of people on ART in the present study. Except for hypercholesterolaemia, the prevalence for most CVD risk factors in the present study is lower than that of ART naïve HIV infected and HIV negative in that study. A high prevalence of CVD risk factors among the general population in this rural area has previously been reported . The fact that people on ART have monthly consultations at clinics and are informed of healthy lifestyles needed to manage co-morbidities could possibly explain the lower prevalence of CVD risk factors compared to ART naïve and HIV negative from same locality. Furthermore majority of participants (96 %) in the present study were receiving NNRTI based regimen associated with lesser CVD risk compared to PI based regimen .
Our study found a 73.8 % level of agreement between the Framingham and DAD risk estimation equations, which was similar to an agreement level of 77.4 % reported by Nery et al. (2013) . Despite this level of agreement observed in our study, the Framingham equation underestimated the risk for CVD in 43 of the 164 participants, when compared to the DAD equation. These results suggest that the use of the Framingham equation in people infected with HIV receiving ART may lead to the exclusion of some individuals to benefit from more aggressive CVD prevention. The low rate of CVD risk as measured by the Framingham equation in our study may be that our cohort was relatively young and predominantly composed of females. Similar to our findings, literature suggests that the Framingham equation underestimates the risk of CVD in South Africans . However, contrary to our findings, the Framingham equation overestimated the 10-year CVD risk among HIV infected Thais  and Brazilians  when compared to DAD equation.
Our study is one of the first that assessed a wide range of CVD risk factors as well as determined the 10-year CVD risk for persons with HIV infection on ART in a rural population in South Africa. Limitations of our study include its cross-sectional design, therefore we cannot conclude that the associations between covariates and CVD risk factors are causal. Information on tobacco use, alcohol use, physical activity and fruit and vegetable intake was obtained using the WHO STEP questionnaire. This is considered to be a reliable instrument, however recall bias may have influenced the results. Non-random sampling was used to recruit participants. However, recruitment was conducted for a whole month cycle, giving all patients collecting their medication equal opportunity to participant. Follow up on participants who failed to turn up for the agreed scheduled date helped to reduce selection bias. While our sample may not be representative of the whole population of HIV infected South Africans receiving ART our study provides valuable and useful information for comparison with other published studies from both developing and developed countries. We also acknowledge the small sample size of our study.
The CVD risk factors were common among this rural population on ART. While 6.7 % of participants had a moderate risk, none had a high risk of developing a CVD event in the next 10 years, according to Framingham risk score equation. However, the high proportion of people with a moderate to very high risk of developing CVD in the next 5 years according to DAD risk score equation clearly represents a considerable health burden that can possibly be rectified by increasing educational programs on CVD prevention for people on ART. Whilst the DAD equation was not developed for Africans, its use instead of the currently used Framingham risk table in people on ART in South Africa, to identify people with a high CVD risk may help to reduce the burden of CVD on the health system. There is a need however to develop and evaluate a race/ethnicity-specific CVD risk estimation tool for HIV infected Africans.
MA, JPV contributed substantially to the conception and design of study. FM collected data, performed analysis, interpretation of data and drafted the manuscript. MA, JPV and RC were involved with the interpretation and presentation of data, and the critical review of the manuscript. All authors read and approved the final manuscript.
We acknowledge the collaboration from ARV treated HIV infected participants recruited from PHC clinics in Dikgale HDSS site. Gratitude is also given to the counsellors and professional nurses for their assistance during recruitment. We are grateful to the Department of Health for granting us permission to conduct the study in Dikgale clinics. We are thankful for the support of the Belgium Development Co-operation through Vlaamse Interuniversitaire Raad-University Development Cooperation (VLIR-UOS) within the framework of an institutional collaboration between the University of Limpopo and Flemish universities.
The authors declare that they have no competing interests.
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