- Open Access
Development of an anti-HIV vaccine eliciting broadly neutralizing antibodies
© The Author(s) 2017
- Received: 7 April 2017
- Accepted: 11 August 2017
- Published: 12 September 2017
The extreme HIV diversity posts a great challenge on development of an effective anti-HIV vaccine. To solve this problem, it is crucial to discover an appropriate immunogens and strategies that are able to prevent the transmission of the diverse viruses that are circulating in the world. Even though there have been a number of broadly neutralizing anti-HIV antibodies (bNAbs) been discovered in recent years, induction of such antibodies to date has only been observed in HIV-1 infection. Here, in this mini review, we review the progress in development of HIV vaccine in eliciting broad immune response, especially production of bNAbs, discuss possible strategies, such as polyvalent sequential vaccination, that facilitates B cell maturation leading to bNAb response.
- Broadly neutralizing antibody
- Polyvalent vaccine
- B cell maturation
According to the WHO, there were ~36.7 million people worldwide living with HIV/AIDS by the end of 2015 and 2.1 million new HIV infections in 2015. In Canada, there were estimated 75,500 people living with HIV infection or AIDS at the end of 2014, a 9.7% increase from 2011, with more than 2500 people are newly infected each year. Unfortunately, even after over 30 years of intensive research, there is still no effective anti-HIV vaccine. This mini review will focus on the development of anti-HIV vaccine targeting elicitation of broadly neutralizing antibodies (bNAbs) against extremely diverse HIV strains.
The extreme genetic diversity of HIV, as a result of high baseline rates of viral mutation and replication, has been a great challenge for HIV vaccine development [1, 2]. There are two types of HIV: HIV-1, predominant throughout the world, and HIV-2, found primarily in West/Central Africa. HIV-1 contains four groups: M (main), O (outlier), N (non-M/non-O), and P (pending). Group M is further subdivided into 9 distinct subtypes  and numerous additional circulating recombinant forms (CRF) . Viruses within the same subtype differ by up to 20%, within the highly variable env region by up to 38%. Furthermore, the virus continuously diversifies in infected individuals, resulting in the virus quasispecies varying up to 5% genetic difference in the same patient at different time points. These quasispecies compose of a unique and highly complex mixture of variants in infected individuals, and ultimately give rise to a highly diverse global virus population.
It is widely thought that an effective strategy to prevent HIV infection will likely come from T cell and B-cell mediated immunity, especially a broadly neutralizing antibody (bNAb) response against the Envelope (Env) protein. The power of bNAbs comes from their ability to recognize epitopes from a variety of viruses, i.e. tackling the extreme viral diversity, and their ability to protect in vivo at low plasma levels . A safe vaccine eliciting bNAbs against HIV could be used to attenuate its spread.
To overcome HIV-1 diversity, one approach is to include different clades to develop a broadly protective polyvalent vaccine. However, early studies on polyvalent vaccine showed inconsistent results regarding elicitation of broad immune responses. Several early studies showed that a polyvalent vaccine, comprising a combination of multiple Env proteins, was better at eliciting broader immune responses than monovalent Env in both rabbits and macaques [5–7], while a clinical phase 2b trial of HVTN505 combining three envelope glycoproteins from clade A, B, and C env genes did not reduce either the rate of acquisition or set point viral load of new HIV-1 infections .
The HIV-1 T cell vaccine field has recently made some significant advances, such as a novel CMV vector developed by Louis Picker et al. , and a polyvalent HIV-1 mosaic antigen strategy which utilizes a genetic algorithm to design small sets of artificial intact viral proteins and collectively optimizes coverage of diverse potential epitopes in a targeted population for a given set size, or valency . Vaccination of a Rhesus Macaque model showed that over 50% of SIVmac infections were effectively cleared in animals that were vaccinated with SIV antigens delivered by the CMV-vectored SIV vaccines . Several recent studies have shown that polyvalent HIV-1 Mosaic antigens result in significantly greater breadth and potency of vaccine-elicited T-cell responses than do natural proteins in NHP studies [10–12].
Characteristics of anti-HIV bNAbs
Neutralization breadth, %
Neutralization potency, μg/ml
Length of CDR H3, a.a.
Somatic mutations %
Year of generation
It has been reported that, during chronic infection, potent and cross-reactive bNAbs that are capable of neutralizing heterologous viruses of diverse subtypes develop in a small portion of HIV-1 infected individuals [28–31]. The effective humoral responses are slow, with NAbs to the initial viral strain appearing after ~12 weeks, and broad NAbs (in 10–30% of individuals) after 2–4 years [30, 32–34]. The development of bNAbs was shown to correlate with high plasma viremia and could result from evolving antigen exposure over many years that has allowed sufficient somatic hypermutation in the B-cell receptors (BCRs) and focuses the B-cell response to the conserved neutralization sites on Env . Therefore, delayed bNAb response might be attributed to the slow, antigen-dependent affinity maturation process. Abs typically accumulate mutations in the complementarity determining region (CDR) loops, i.e. the typical antigen contact region . Whereas most human Abs that have undergone affinity maturation carry 15–20 somatic mutations, potent anti-HIV bNAbs carry 40–110 mutations. Reversion of these mutations to the germline sequence drastically reduces their neutralizing potency and breadth [36–40]. These findings suggest that bNAb-producing B cells are the products of clonal evolution which coined the term “B cell maturation”. Thus, selecting the right combination of immunogens and vaccination strategy is crucial for induction of these bNAb-producing B cells via continual increase in affinity-driven selection in the germinal centers (GCs).
A vaccine strategy that aims to mimic the diverse antigenic exposure experienced during natural infection may generate NAbs of greater breadth and potency. Morner et al. achieved greater focusing of the immune response on conserved regions of Env by sequential vaccination of macaques with gp120 core protein followed by trimer boosting . Similarly, the administration of sequential rather than single or mixed patient-derived gp140 Env genes in rabbits showed a marginal improvement in breadth of neutralization . Sellhorn et al. recently described a novel assembly of gp140 heterotrimers which had gp140 subunits from two different genetic sources and improved potency of NAb responses in rabbits as compared to homotrimeric equivalents . Wang et al. also showed through vaccinating mice with three gp120 variants that sequential vaccination is preferred over a cocktail for induction of cross-reactive Abs focused on the shared CD4 binding site epitope .
We have tested our polyvalent anti-HIV vaccine in a human CD4 B cell transgenic mouse model which was established to express human CD4 receptor on the surface of B cells, thus can mediate HIV binding, and expose the hidden epitopes on viral gp120. We have tested 25 primary isolates (subtype A, B, C, and D) derived functional Envs and 25 nonfunctional inter-subtype recombinant Env-based VLP vaccines in the human CD4 B cell transgenic mice. The results showed that the sequential vaccination with the 25 functional primary Env-based polyvalent vaccine elicited broader NAb response than any other vaccine combination and vaccination strategy when examining inhibition of a number tier 1 and 2 viruses with different HIV-1 subtype Envs (e.g. A, B, C, and D). More importantly, with each increase in the diversity and number of vaccines (i.e. sequential vaccination), we observed a greater breadth in humoral responses (unpublished data). These results suggest that continuous stimulation with diverse HIV-1 Env immunogens will modulate B cell affinity maturation, educate the immune response to focus on certain regions (most likely on the conserved regions) in Env, and finally have the best success of generating broad NAb response.
A major obstacle for HIV vaccine development is the extreme virus diversity. Although a number of bNAbs have been isolated from HIV patients, the vaccine and procedures capable to elicit such a response remain a mystery. The sequential vaccination with multiple immunogen variants might favor B cell re-circulation within GCs for additional rounds of affinity maturation. This may promote the B cell response to focus on the most conserved regions of immunogens through positive selection, resulting in more potent and broader antibody responses.
All authors contributed to writing and reviewing the manuscript. All authors read and approved the final manuscript.
Work in the corresponding author’s laboratory was supported by a Canadian Institute of Health Research Grant (HBF143165), and NIAID, NIH (R0184816).
The authors declare that they have no competing interests.
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