Fig. 1
From: Development of an anti-HIV vaccine eliciting broadly neutralizing antibodies
SHIVenv vaccine system. a The vaccine vector is derived from 293T transfections with three DNA plasmids: 1 pREC_SHIVKB9 _gag/pol vector containing gag and pol coding sequence from SHIVKB9 but lacking the LTRs or RNA packaging elements; 2 pREC_SHIVKB9_env_Δgagpol vector which contains the RNA packaging signal, env and 3′LTR, but lacks 5′LTR, gag and pol sequences; 3 pCMV_SHIVKB9_ cpltRU5 which contains only the 5′LTR, primer binding site, and the RNA packaging signal. b Generation of SHIVenv virus-like particles as HIV vaccine candidate through triple transfection. c Production of defective SHIV-1 proviral DNA and Env glycoproteins by pseudotyped virus containing the two complementary subgenomic viral RNAs