Determinants to antiretroviral treatment non-adherence among adult HIV/AIDS patients in northern Ethiopia
© The Author(s) 2017
Received: 25 October 2016
Accepted: 11 March 2017
Published: 21 March 2017
Adhering 95% and above of antiretroviral therapy reduces the rate of disease progression and death among people’s living human immunodeficiency virus. Though manifold factors have reported as determinant factors of antiretroviral therapy adherence status, perhaps determinants of non-adherence differ up on the activities of patients in the study setting.
An institution based unmatched case–control study was conducted in Aksum town. Individuals who had a 6-month follow-up with complete individual information were included in the study. Document review and interviewer based techniques were used to collect the data. Binary logistic regression analysis was used to identify the determinant factors of non-adherence.
A total of 411 (137 cases and 274 control) study participants were included in the study. The majority of them were male in sex. Having 2 years and above duration on ART [AOR = 7, 95% CI (2.2, 22.6)], history of adverse effect [AOR = 6.9, 95% CI (1.4, 32.9)], substance use [AOR = 5.3, 95% CI (1.4, 20.0)], living with parents [AOR = 3.4, 95% CI (1.2, 10.3)], having depression symptom [AOR = 3.3, 95% CI (1.4, 7.5)], <350 cells/mm3 cluster of differentiation 4 count [AOR = 3.2, 95% CI (1.8, 5.8)] and low dietary diversity [AOR = 2, 95% CI (1.1, 3.7)] were found significant determinants of non-adherence to antiretroviral drug.
Program, social and individual related factors showed a statistically significant associated with non-adherence to antiretroviral therapy. Managing lifestyle by developing self-efficacy of individuals and treating related threat to improve adherence status of antiretroviral therapy is recommended in this study.
In 2013 globally, 35 million people living with Human Immunodeficiency Virus (HIV). Worthily, sub-Saharan Africa (23.5–26.1 million) people living with HIV which is 71% of the global prevalence UNAIDS . Ethiopia, an estimated 793,700 (716,300–893,200) people living with HIV/AIDS .
Antiretroviral therapy (ART) is a proven treatment for HIV/ADS patients in improving the health status and quality of life of HIV/AIDS patients by reducing the rate of disease progression . Appropriately taking of the treatment is the advisable option in order to obtain full benefits of ART; durable suppression of viral replication, reduced destruction of CD4 cells, prevention of viral resistance, promotion of immune reconstitution, and slowed disease progression . Poor ART adherence is a notable public health problem in developing countries .
An individual considered as non-adherence for ART if he/she had a history of taking doses 2 or more hours before, and/or 2 or more hours after the time of a doctor’s advice to take doses or missing doses completely (i.e., <95% adherence = missing >2 doses of 30 doses or >3 doses of 60 doses) [5, 6]. In 2012, over 9.7 million people living with HIV in low- and middle-income countries were receiving ART, however, ensuring adherence to HIV treatment remains challenging in all countries .
Adherence of ART is a complex behavior, which is influenced by several determinants, majorly patient loss to follow-up and ensuring adherence to ART regimens remain major challenges in Ethiopia [2, 5].
Variables such as; availability of reminder, substance use, malnutrition, dietary diversity, CD4 count, depression symptom, adverse effect of ART and duration on ART were reported as a determinant factor for non-adherence to ART [5, 6, 8–12]. Duration on ART [13, 14] and CD4 count in [11, 15] were associated with non-adherence to ART. Determinant factors for non-adherence are multiple and have different effects. This study was aimed to identify determinants of non-adherence to ART among HIV-infected adults in Aksum town health facilities, northern Ethiopia.
An institution based unmatched case–control study was carried out from March 20 to May 15, 2015, in Aksum Health Center and Aksum Hospital, Aksum, Ethiopia. Aksum town is located 1067 km away in Northeast Ethiopia of Addis Ababa, the capital city of Ethiopia.
Sample size and sampling procedure
The sample size of the study was calculated by Epi-Info™ 7 software Statcalc program using the following assumptions; proportion of non-adherence among not exposed (controls) 12.1%, proportion of non-adherence among exposed (cases) 21.7%, odds ratio 2.18 , 5% level of significance, 80% power of the study and 1:2 case to control ratio.
All HIV/AIDS positive adults (18+ years old) who had at least three consecutive visits before a data collection period were considered as a study population of this study. The baseline data was collected from two health facilities using clinical record review. For the present study, study groups based on ART adherence status was defined as cases; patients who had a history of greater 5% (missing >2 doses out of 30 doses or >3 doses out of 60 doses) all of scheduled regimens which supposed to take in a month and controls were near-perfect (>95%) take to dose of all regimens which supposed to take in a month [17, 18]. All cases who had to follow-up during the data collection period were included in the study whereas controls were included by systematic random sampling techniques in every other four appointees.
Data collection and data analysis
The data were collected using chart review and interviewer administered techniques by two diploma nurses and three case managers working in ART clinics supervised by two bachelor nurse professionals. The structured questionnaire consisted of socio-demographic, socio-economic, nutritional, medical and psychological were used for the data collection tool. A 1-day training on the objective of the study, confidentiality of information and interviewing techniques were given. Baseline information was filled out from ART registration and follow-up forms.
Dietary diversity was assessed by asking the number of reported different foods and food groups consumed in a household over a 24-h period of the data collection time, does not include food group consumed outside the home. It was categorized into three groups; low if <3 food items consumed, medium if 4–6 food items consumed and high if ≥6 food items consumed .
Patient Health Questionnaire (PHQ) with 9-items on which score ranges from 0 to 27 were used to assess the depression of the study participants. Individuals up on their response categorized into five categories as (no depression, mild depression, moderate depression, moderately severe depression and severe depression) .
Precoding and manual checking of the questionnaire were done by the principal investigator before the data entry. Data was entered using Epi-Info and exported to SPSS version 20 for further cleaning and analysis purpose. Binary logistic regression model used to identify the determinant factors. Collinearity diagnostic test was conducted using tolerance to check for co-linearity between independent variables and interaction effect. Variables in the bivariable analysis having a p value <0.2 were considered for multivariate analysis to adjust the confounders. The strength and presence of statistical association were assessed by OR, p value and 95% confidence interval (95% CI). Variables with a p value ≤0.05 were considered as statistically significant determinant factors of non-adherence to ART. Hosmer–Lemeshow goodness-of-fit test (p = 0.9289) was used to assess the fitness of the model.
Socio-demographic characteristics at the time of presentation and during data collection time of cases and controls, Aksum town, northern Ethiopia 2015
Frequency of adherence status
Case, n (%)
Control, n (%)
Educational level (grade)
No formal education
Primary education (1–8)
Secondary education (9–12)
Determinants of non-adherence to antiretroviral therapy
In multivariable analysis variables, such as duration of treatment, ART adverse effect, substance use, living condition, depression, CD4 cell count and dietary diversity were found to be independent determinant factors with non-adherence to ART.
Patients who had more than 2 years ART follow-up [AOR = 7, 95% CI (2.2, 22.6)] were 7 times more likely to not adhere as compared to those who had 6–12 months follow-up. Patients who had a history of ART adverse drug effect [AOR = 6.9, 95% CI (1.4, 32.9)] were 6.9 times more likely to be non-adherent as compare to the counterpart. HIV-positive substance user [AOR = 5.3, 95% CI (1.4, 20)] individuals who were 5.3 times more likely to be non-adherence as compared with the counterpart.
Non-adherence were 3.4 times more likely to occur among those who live with their parents [AOR = 3.4, 95% CI (1.2, 10.3)] as compared to those who live alone. This study also showed that depression is associated with non-adherence of ART. The odds of non-adherence were 3.3 times more likely to those who had depression symptom [AOR = 3.3, 95% CI (1.4, 7.5)] as compared to their counterparts.
Binary and multiple logistic regression model showing determinants of non-adherence to antiretroviral therapy, among adults on ART Aksum town, northern Ethiopia 2015
Adherence status to ART
COR (95% CI)
AOR (95% CI)
Non-adherent number (%)
Adherent number (%)
Duration on ART (months)
3.2 (1.4 7.4)
ART adverse effect
CD4 cell count (cells/mm3)
Medium and above
Daily eating pattern
Stage II and above
Treatment other than ART
ART has a proven clinical benefit in HIV-infected people by slow downing the progressing to AIDS. Incomplete adherence to the treatment leads to immune suppression and the selection drug resistant HIV . Non-adherence increases HIV-related morbidity and mortality . Numerous studies were conducted on the determinants of ART drug-adherence and/or non-adherence of HIV/AIDS patients in the country. However, studies signify the consequence of non-adherence is one or more factors that may have different effects consequently; the study setting, socio-demographic characteristics of the study participants, and sample size. This study showed that having 2 years and above duration on ART, depression symptoms, history of ART adverse effect, substance use, living with parents, <350 cells/mm3 CD4 count and low dietary diversity in the household as determinant factors for non-adherence to ART.
Coherent with the previous studies in Ethiopia [13, 23] HIV-positive participants who had more than 2 years ART follow-up were not taken ART drugs appropriately. This finding was also coherent with the Asia study conducted in Vientiane, Lao PDR . Findings of Asia [25, 26] inconsistent with the present study, patients who have been on ART for short period were more likely to be non-adherent. The possible reason for the discrepancy could be these studies were included those patients who had less than 6 months’ duration on ART since ART at an early phase of treatment may have severed adverse drug effects. On top of the treatment duration of the patients, the accessibility of health facilities, living conditions, socio-demographic characteristics of the population in Asia is differ from Ethiopia which might have effect on the adherence status of the patient.
Like the report of previous studies conducted in Ethiopia  and in Nepal  in the present study, participants that had experience of adverse drug effect during their medication were more likely to non-adherence to ART. But, this study is not consistent with a systematic review of Asian developing countries in 2012 reported . The possible explanation will be they already counselled on the possible side effects of the drug during their medication follow-up time.
Substance uses among patients during ART medication was also found to be the independent determinant factors of non-adherence, a finding consistent with a study done in southern Ethiopia , Tanzania  and Nepal .
In the present study, the living conditions are a determinant factor of non-adherence. Patients living with their families were more likely to non-adherence of ART drug as compared to those living independent from their families, consistent with previous study done in southern Ethiopia  inconsistent with a study done in Canada  and Cambodia . The difference may be due to clients’ self-efficacy and attitude towards ART medication is stumpy in the study setting, even at a country level.
The presence of depressive symptoms during medication is also reported as one of determinant factor for ART drug adherence, supported with studies done in South-west Ethiopia [14, 29], South Africa  and Netherlands . Possibly depressed patients could easily forget to take their dose while they managed the depression symptoms.
Similar to other studies conducted in Ethiopia [15, 34, 35], and in Africa [32, 36] the level of CD4 count during initiation of treatment have independent effect for adherence status. Non-adherence was higher among patients with a CD4 count <350 cells/mm3 compared to their counterparts. The finding is supported by studies in Ethiopia [15, 34, 35] however, a finding reported in Yirgalem Hospital, southern Ethiopia  was not consistent with the present study. This may be due to the previous study controlling for both WHO stages and CD4 cell count for the initiation criteria of ART.
Moreover, low dietary habits of the patient are reported as the independent determinant factors of non-adherence. This finding is supported by reports in South and North Ethiopia [15, 16] and Zambia .
Unlike previous studies, socio-demographic factors such as sex, age, residence, occupation, eating pattern and patient level factors; didn’t show statistically significant association with non-adherence of ART. This may be because of in this locales the barriers of non-adherence were beyond the listed variables.
Since identifications of case and controls were done based on the registration log book and individual chart review, this paper may be prone to misclassification bias. Because of the adherence status of the patient were assessed by patient self-report, the non-adherence status may underestimate. Moreover, information bias was also a problem while assessing dietary diversity, substance use and depression symptom.
Having more than 2-year duration on ART follow-up, experiencing, adverse ART drug effect, substance use, living with parents, having depression symptom, CD4 cell count <350 cells/mm3 and low dietary diversity were all found independent determinant factors of non-adherence of ART drug. Defining the adherence status with viral load suppression and/or measured by detectable viral load during chronic care and identifying the treatment outcome of non-adherence patient is recommended for future researcher.
BBG, YK, YAG, MKY, DT and MA equally participated in this study. All authors read and approved the final manuscript.
Aksum Hospital and Health Center staff, study participants, data collectors and supervisors deserve acknowledgement for their unreserved support.
BBG, YK, and YAG participated from objective formulation to manuscript drafting. MK, DT and MA are participated during data analysis and manuscript drafting. All authors shared professional contribution for this study.
Availability of data and materials
All data underlying the findings are fully available online without restriction. Data are available in figure share: https://figshare.com/s/ca4e9a1ae825395ba236.
Ethics approval and consent to participate
The study was conducted after receiving ethical approval from Intuitional Review Committee of the University of Gondar, college of Medicine and Health Science, Institute of Public Health. Before the ethical approval, the proposal was provided to academic reviewers to assure the ethical issues. Finally, the ethical review committee approved the oral consent. Permission letter was obtained from the respective health institutions (Aksum Town Health Office, Aksum Health Center and Aksum Hospital) persons in charge and from ART Clinic nurses and data managers. Study participants were informed of the purpose of the study and obtained written informed consent. To ensure confidentiality, names were not mentioned in the data collection tools and result reports.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- UNAIDS. The Gap Report Switzerland Geneva updated Sept 2014. July 2014.Google Scholar
- HIV AIDS Prevention and Control Organization. Federal Democratic Republic of Ethiopia country progress report on the HIV response. Addis Ababa:MOH; 2014.Google Scholar
- Joint United Nations Programme on HIV/AIDS. United Nation AIDS world AIDS day report 2011 (Faster, Smarter, Better). Geneva:UNAIDS; 2011.Google Scholar
- Steel G, Nwokike J, Joshi MP. Development of a multi-method tool to measure ART adherence in resource-constrained settings: the South Africa experience. RPM Plus. Arlington, VA: Management Sciences for Health; 2007.Google Scholar
- Asmare M, Aychiluhem M, Ayana M, Jara D. Level of ART adherence and associated factors among HIV sero-positive adult on highly active antiretroviral therapy in Debre Markos Referral Hospital, Northwest Ethiopia. J Antivir Antiretrovir. 2014;6:120–6. doi:10.4172/jaa.10000107.Google Scholar
- Sarna A, Kellerman S. Access to antiretroviral therapy for adults and children with HIV infection in developing countries: horizons studies, 2002–2008. Public Health Rep. 2010;125(2):305–15.PubMedPubMed CentralGoogle Scholar
- The World Health Organization. Global update on HIV treatment: results, impact and opportunities. 2013.Google Scholar
- Mills EJ, et al. Adherence to antiretroviral therapy in sub-Saharan Africa and North America: a meta-analysis. JAMA. 2006;296(6):679–90.View ArticlePubMedGoogle Scholar
- Stein MD, et al. Adherence to antiretroviral therapy among HIV-infected methadone patients: effect of ongoing illicit drug use. Am J Drug Alcohol Abuse. 2000;26(2):195–205.View ArticlePubMedGoogle Scholar
- Ortego C, et al. Adherence to highly active antiretroviral therapy (HAART): a meta-analysis. AIDS Behav. 2011;15(7):1381–96.View ArticlePubMedGoogle Scholar
- Tessema B, et al. Magnitude and determinants of non-adherence and nonreadiness to highly active antiretroviral therapy among people living with HIV/AIDS in Northwest Ethiopia: a cross-sectional study. AIDS Res Ther. 2010;7(1):1.View ArticleGoogle Scholar
- Ethiopian Federal Minstry of Health. National Guidelines for Comprehensive HIV Prevention, Care and Treatment. Addis Ababa: Ethiopian Federal Minstry of Health; 2014.Google Scholar
- Mitiku H, Abdosh T, Teklemariam Z. Factors affecting adherence to antiretroviral treatment in Harari National Regional State, eastern Ethiopia. ISRN AIDS. 2013;. doi:10.1155/2013/960954.Google Scholar
- Amberbir A, et al. Predictors of adherence to antiretroviral therapy among HIV-infected persons: a prospective study in Southwest Ethiopia. BMC Public Health. 2008;8(1):1.View ArticleGoogle Scholar
- Berhe N, Tegabu D, Alemayehu M. Effect of nutritional factors on adherence to antiretroviral therapy among HIV-infected adults: a case control study in northern Ethiopia. BMC Infect Dis. 2013;13(1):1.View ArticleGoogle Scholar
- Bitew BD, et al. Determinants of none-adherence to antiretroviral therapy among HIV-infected adults in Arba Minch General Hospital, Gamo Gofa Zone, southern Ethiopia: a case control study. Am J Health Res. 2014;5(2):234–40.View ArticleGoogle Scholar
- Kaplan JE, Benson C, Holmes KK, Brooks JT, Pau A, Masur H, Centers for Disease Control and Prevention (CDC), National Institutes of Health, HIV Medicine Association of the Infectious Diseases Society of America. Guidelines for prevention and treatment of opportunistic infections in HIV-infected adults and adolescents. MMWR Recomm Rep. 2009;58(RR-4):1–207.Google Scholar
- Machtinger EL, Bangsberg DR. Adherence to HIV antiretroviral therapy HIV InSite Knowledge Base chapter. 2006.Google Scholar
- De Matteis A, et al. Baseline survey of food security and nutrition in Mozambique. Maputo: Technical Secretariat for Food Security and Nutrition: Vulnerability Analysis Group; 2006.Google Scholar
- Kroenke K, Spitzer RL. The PHQ-9: a new depression diagnostic and severity measure. Psychiatr Ann. 2002;32(9):1–7.View ArticleGoogle Scholar
- Bangsberg DR, Moss AR, Deeks SG. Paradoxes of adherence and drug resistance to HIV antiretroviral therapy. J Antimicrob Chemother. 2004;53(5):696–9.View ArticlePubMedGoogle Scholar
- Haddad PM, Brain C, Scott J. Nonadherence with antipsychotic medication in schizophrenia: challenges and management strategies. Patient Relat Outcome Meas. 2014;5:43–62.View ArticlePubMedPubMed CentralGoogle Scholar
- Irano ZA, et al. Determinants of non-adherence to antiretroviral therapy and modeling progression of adherence level: at Yirgalem Regional Hospital, Ethiopia; January, 2012. Int J Med Health Sci Res. 2015;2(1):1–14.View ArticleGoogle Scholar
- Hansana V, et al. Adherence to antiretroviral therapy (ART) among people living with HIV (PLHIV): a cross-sectional survey to measure in Lao PDR. BMC Public Health. 2013;13:617.View ArticlePubMedPubMed CentralGoogle Scholar
- Jiamsakul A, Kumarasamy N, Ditangco R, Li PC, Phanuphak P, Sirisanthana T, Sungkanuparph S, Kantipong P, Lee CK, Mustafa M, Merati T. Factors associated with suboptimal adherence to antiretroviral therapy in Asia. J Int AIDS Soc. 2014;17(1):18911. doi:10.7448/IAS.17.1.18911.PubMedPubMed CentralGoogle Scholar
- Wasti SP, et al. Factors influencing adherence to antiretroviral treatment in Nepal: a mixed-methods study. PLoS ONE. 2012;7(5):e35547.View ArticlePubMedPubMed CentralGoogle Scholar
- Wasti SP, et al. Factors influencing adherence to antiretroviral treatment in Asian developing countries: a systematic review. Tropl Med Int Health. 2012;17(1):71–81.View ArticleGoogle Scholar
- Idindili B, Jullu B, Mugusi F, Tanner M. A case-control study of factors associated with non-adherent to antiretroviral therapy among HIV infected people in Pwani Region, eastern Tanzania. Tanzania J Health Res. 2012;14(3):194–203.View ArticleGoogle Scholar
- Alagaw A, et al. Factors associated with antiretroviral treatment adherence among adult patients in Wolaita Soddo Hospital, Wolaita Zone, southern Ethiopia. Sci J Public Health. 2014;2:69–77.Google Scholar
- Godin G, et al. Prediction of adherence to antiretroviral therapy: a one-year longitudinal study. AIDS care. 2005;17(4):493–504.View ArticlePubMedGoogle Scholar
- Spire B, et al. Adherence to antiretroviral therapy in patients enrolled in a comprehensive care program in Cambodia: a 24-month follow-up assessment. Antivir Ther. 2008;13(5):697–703.PubMedGoogle Scholar
- Peltzer K, et al. Antiretroviral treatment adherence among HIV patients in KwaZulu-Natal, South Africa. BMC Public Health. 2010;10(1):1.View ArticleGoogle Scholar
- Langebeek N, et al. Predictors and correlates of adherence to combination antiretroviral therapy (ART) for chronic HIV infection: a meta-analysis. BMC Med. 2014;12(1):142.View ArticlePubMedPubMed CentralGoogle Scholar
- Biressaw S, et al. Adherence to antiretroviral therapy and associated factors among HIV infected children in Ethiopia: unannounced home-based pill count versus caregivers’ report. BMC Pediatr. 2013;13(1):132.View ArticlePubMedPubMed CentralGoogle Scholar
- Amberbir A, et al. Predictors of adherence to antiretroviral therapy among HIV-infected persons: a prospective study in Southwest Ethiopia. BMC Public Health. 2008;8(1):265.View ArticlePubMedPubMed CentralGoogle Scholar
- Mbuagbaw L, et al. Trends and determining factors associated with adherence to antiretroviral therapy (ART) in Cameroon: a systematic review and analysis of the CAMPS trial. AIDS Res Ther. 2012;9(1):1.View ArticleGoogle Scholar
- Sasaki Y, et al. Adherence to antiretroviral therapy (ART) during the early months of treatment in rural Zambia: influence of demographic characteristics and social surroundings of patients. Ann Clin Microbiol Antimicrob. 2012;11(1):1.View ArticleGoogle Scholar