Lipoprotein levels and cardiovascular risk in HIV-infected and uninfected Rwandan women
© Anastos et al; licensee BioMed Central Ltd. 2010
Received: 6 June 2010
Accepted: 26 August 2010
Published: 26 August 2010
Lipoprotein profiles in HIV-infected African women have not been well described. We assessed associations of lipoprotein levels and cardiovascular risk with HIV-infection and CD4 count in Rwandan women.
Cross-sectional study of 824 (218 HIV-negative, 606 HIV+) Rwandan women. Body composition by body impedance analysis, CD4 count, and fasting serum total cholesterol (total-C), triglycerides (TG) and high-density lipoprotein (HDL) levels were measured. Low-density lipoprotein (LDL) was calculated from Friedewald equation if TG < 400 and measured directly if TG ≥ 400 mg/dl.
BMI was similar in HIV+ and -negative women, < 1% were diabetic, and HIV+ women were younger. In multivariate models LDL was not associated with HIV-serostatus. HDL was lower in HIV+ women (44 vs. 54 mg/dL, p < 0.0001) with no significant difference by CD4 count (p = 0.13). HIV serostatus (p = 0.005) and among HIV+ women lower CD4 count (p = 0.04) were associated with higher TG. BMI was independently associated with higher LDL (p = 0.01), and higher total body fat was strongly associated with higher total-C and LDL. Framingham risk scores were < 2% in both groups.
In this cohort of non-obese African women HDL and TG, but not LDL, were adversely associated with HIV infection. As HDL is a strong predictor of cardiovascular (CV) events in women, this HIV-associated difference may confer increased risk for CV disease in HIV-infected women.
Dyslipidemias have been described since 1989 in individuals with human immunodeficiency virus (HIV) infection in resource-replete countries, prior to the availability of combination antiretroviral therapy (cART), in studies primarily of white men [1–6]. Quantitative abnormalities include higher triglyceride levels (TG) [2–6] and lower levels of total cholesterol (total-C) [1, 5–7], high-density lipoprotein (HDL) [3, 6, 8] and low-density lipoprotein (LDL) [1–7, 9] cholesterol. These abnormalities are greater with more advanced immune suppression [2–6]. Patterns of lipoprotein changes are somewhat different in HIV-infected women , with most studies showing no association of HIV-infection with lower LDL. Both women and African Americans in general have higher HDL and lower TG than do European American men [11, 12]. Further information is required regarding lipoprotein profiles and potential cardiovascular risk in HIV-infected Africans, who start with lower total-C, TG and LDL, and higher HDL, compared to individuals of European descent living in resource-replete settings. A South African study found lower HDL and LDL and higher TG in HIV-infected compared to uninfected patients , and a study in Uganda has reported little change in lipoprotein levels over a two-year period in patients initiating cART .
In sub-Saharan Africa and the United States, women and/or people of African descent represent the majority of those infected with and treated for HIV-infection. Because cardiovascular disease (CVD) is a growing concern for HIV infected individuals [15, 16] and in developing countries, and dyslipidemia is a major risk factor for CVD, we assessed cardiovascular risk and predictors of lipoprotein levels in HIV-infected and uninfected Rwandan women.
The Rwanda Women's Interassociation Study and Assessment (RWISA) is an observational prospective cohort study of HIV-infected and uninfected Rwandan women investigating the effectiveness and toxicity of antiretroviral therapy. In 2005, 710 HIV-infected and 226 HIV-uninfected women were enrolled with follow-up visits occurring every six months. Informed consent was obtained in accordance with protocols approved by the Rwandan National Ethics Committee and the Institutional Review Board of Montefiore Medical Center, Bronx NY, USA. Participants were recruited through grassroots women's organizations and clinical care sites for HIV-infected patients. Inclusion criteria were: age 25 years or older at study entry, willingness to give informed consent, presence in Rwanda during 1994 and no history of receiving antiretroviral treatment except single dose nevirapine to prevent mother-to-child transmission of HIV. At study entry participants provided historical information including socio-demographics, medical and psychosocial history and symptoms, and experience of trauma during the 1994 Rwandan genocide. A physical examination and body impedance analysis (BIA) were performed, and fasting blood specimens were taken. This cross-sectional analysis includes enrollment data from 824 participants for whom fasting lipoprotein levels were measured at study enrollment.
Total-C, triglyceride and HDL analysis and albumin determination (as a proxy biochemical measure of nutritional status), were performed in the laboratory of King Faisal Hospital in Kigali, Rwanda using standard laboratory methods. For 112 women lipid values were not available because of a lack of reagents at the time of their study visits. LDL was calculated with the Friedewald equation , using measured values for total cholesterol, HDL and triglycerides. [LDL = (total-C) - (HDL) - (Trig/5.0)]. If triglyceride level was ≥ 400 mg/dL (n = 11), LDL was measured directly by Quest Laboratory in Baltimore Maryland USA. CD4 counts were determined with a FACS counter (Becton and Dickinson, Immunocytometry Systems, San Jose, CA, USA) at the National Reference Laboratory of Rwanda.
The primary exposure variables were HIV serostatus (positive vs. negative) and in the HIV-positive women CD4 cell count, both as a continuous variable (per 100 cells/μl increment) and categorized as < 200, 200-350, and > 350 cells/μl. Secondary exposure variables included age, income, educational attainment, body mass index (BMI) calculated as weight in kilograms divided by (height in meters)2, total body fat (TBF) and lean body mass calculated from BIA body composition measures and weight, self-reported menopausal status, current tobacco smoking (yes vs. no), history of diabetes mellitus defined by self-report or fasting plasma glucose > 125 mg/dL, and a reported perception of having adequate food. HIV-1 RNA was included for the 271 HIV+ women whose value was measured.
The primary outcomes of interest were total-C, LDL, HDL, and TG, and calculated Framingham 10-year CVD risk.
Analysis of variance or chi-square tests were used to compare mean lipid levels and percent of participants with abnormal lipid levels among groups defined by HIV status and CD4 count. Variables included in the multivariate models were chosen by their association with lipoprotein values in univariate analysis; variables with p < 0.10 were included in the multivariable models analyzing the outcome variables among the groups. If the overall ANOVA test for differences in lipid levels among > 2 groups was significant, two-sample tests were used to assess pair-wise comparisons. Otherwise, pair-wise comparisons are not reported. In assessment of the Framingham Risk Score (FRS) we performed an age-matched analysis, with each pair randomly selected with the age-difference within +/- 2 years. We also calculated FRS without including the age component. All analyses were performed using the SAS statistical package (Version 8, SAS Institute, Cary, NC).
Participant Characteristics, Unadjusted Lipoprotein Levels, by HIV Serostatus and CD4 Count
N = 218
HIV+ women (all)
N = 606
HIV+ CD4 > 350
n = 158
HIV+ CD4 200-350
n = 224
n = 222
Age (years) Mean (SD)
Body Mass Index (Kg/m 2 )
Total Body Fat (Kg)
Lean Body Mass (Kg)
# Sexual partners 1
2 (1, 4)
3 (2, 6)
3 (2, 5)
3 (2, 5)
3 (2, 6)
> 3 drinks/day
Illicit drug use (%)
Food adequacy (%)
Most of the time
Some of the time
Running water in home (%)
Completed high school
Monthly Income $US
Diabetes Mellitus (%)
CD4 cell count (cells/mm 3 )
HIV-1 RNA* (log 10 )
n = 271
n = 34
n = 93
n = 143
Lipoprotein Levels, mg/dl
Associations with HIV serostatus and CD4 cell count
Multivariate associations of Lipoprotein levels with HIV serostatus and CD4 lymphocyte count in 824 participants
Lipoprotein LevelsMean (Standard error)
N = 218
N = 606
HIV+ CD4 > 350
N = 158
N = 224
CD4 < 200
N = 222
Total Cholesterol 5
Associations of lipid levels with demographic and clinical variables and measures of nutritional status
Quartiles of total body fat adjusted for CD4 lymphocyte count in HIV-positive women
Mean (Standard Deviation)
Total Body Fat in Kilograms
7.4 to 11.8
11.8 to 17.4
Framingham Risk Scores
Framingham 10-year risk scores by HIV-serostatus
N = 218
N = 606
Framingham 10-year risk (FRS)
FRS without age component
FRS age-matched (n = 308)
In this study of HIV-negative and ART-naïve HIV+ Rwandan women we found associations of serum lipoprotein levels with HIV serostatus and CD4 count that were similar to those described in United States women . HDL was markedly lower in the HIV+ women with some variation by CD4 count, with values very similar to those in US women, and TG levels were higher with HIV+ serostatus and advanced immune suppression. As in US women, but unlike US men, LDL levels did not differ significantly by HIV serostatus or CD4 count [9, 10]. The weak association of lower total-C in HIV+ women was entirely explained by the lower HDL. Unlike HDL, in our study LDL, TG and total-C were not similar to values in US women--all were markedly lower.
The clinical importance of abnormal lipoprotein levels lies primarily in the greater risk they confer for development of CVD [18, 19], and there is growing concern about premature CVD in both untreated and cART-treated HIV+ persons [15, 16]. However, the lipoprotein profiles of the HIV-negative RWISA participants are quite favorable by United States standards, and the changes associated with HIV-infection may thus not be clinically significant. Still, subclinical atherosclerosis has been found to be more common in HIV+ men independent of FRS  and higher HDL is a powerful protective factor for cardiovascular disease in women, of greater importance than it is in men [21, 22]. The very low HDL in untreated HIV+ women and the moderately higher TG levels may indeed increase CVD risk in African women, and further information is needed to determine whether clinical CVD outcomes will increase with HIV treatment. Only 15% of HIV-negative and 11% of HIV+ women had LDL levels above 100 mg/dL, the most stringent target when treating LDL levels in the United States.
Some cART regimens are associated with worse lipoprotein profiles, especially protease inhibitors (PI) [9, 23, 24] and stavudine [25, 26]. The non-nucleoside reverse transcriptase inhibitors (NNRTI) have been associated with beneficial effects on HDL [24, 25]. The NNRTIs are the most common anchor drugs in cART regimens in Africa, and stavudine is increasingly avoided. Thus African women initiating ART may benefit with improved lipoprotein profiles, especially higher HDL. Follow-up of Africans initiating cART will improve our understanding of its effects on lipoprotein levels, potential CVD risk, and the actual rates of CVD events.
We found that the total body fat did not impact meaningfully on the HDL and TG values associated with HIV disease. However, TBF was significantly associated with higher LDL, non-HDL and total cholesterol. The trends in associations of lipid levels with TBF were similar in the HIV+ and negative women. Thus body composition was not informative in assessing the impact of HIV immune suppression.
Limitations of our study include its cross-sectional design and the absence of information on diet and exercise. Strengths include the relatively large sample size, the uniformity of specimen and data collection, and the inclusion of body composition measures.
We found that lower HDL and higher TG were the primary adverse lipoprotein levels associated with HIV infection in Rwandan women, while LDL was less affected. Because HDL is a strong predictor of CVD outcomes in women, this HIV-associated difference may carry significant risk of CVD in HIV-infected women. Longitudinal studies of African women receiving cART are needed to define these patterns in the presence of HIV treatment and to ascertain the associated cardiovascular risk.
The authors thank the study participants and staff of RWISA, especially Bosco Ndabarinze. The authors dedicate this paper to the memory of their colleague Dr. François Ndamage, RWISA Principal Investigator 2007-8.
This study was supported by supplements from the National Institute of Allergy and Infectious Diseases to the Bronx/Manhattan Women's Interagency HIV Study (WIHS), which is funded by the National Institute of Allergy and Infectious Diseases (UO1-AI-35004). This work was also supported in part by the AIDS International Training and Research Program (Fogarty International Center, NIH D43-TW001403) and the Center for AIDS Research of the Albert Einstein College of Medicine and Montefiore Medical Center funded by the National Institutes of Health (NIH AI-51519) and by the National Institute of Diabetes and Digestive and Kidney Disease (DK54615), and the Chicago WIHS (U01-AI-34993).
Data in this manuscript were presented in part at the XVI International AIDS Conference, Toronto, Canada; August 13-18, 2006.
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