The prevalence of transmitted HIV drug resistance among MSM in Anhui province, China
- Yuelan Shen†1,
- Bin Su†1Email author,
- Jianjun Wu1,
- Yizu Qin1,
- Lin Jin1,
- Lifeng Miao1,
- Aiwen Liu1 and
- Xiaoli Cheng1
© Shen et al.; licensee BioMed Central Ltd. 2014
Received: 21 April 2014
Accepted: 4 July 2014
Published: 8 July 2014
To optimize treatment regimens, we assessed human immunodeficiency virus (HIV) diversity and the prevalence of transmitted drug resistance (TDR) among men who have sex with men (MSM) in Anhui province, China.
A total of 139 MSM who were newly diagnosed and antiretroviral treatment-naive were enrolled in Anhui in 2011. A partial pol fragment was amplified and sequenced, and HIV subtypes were determined by phylogenetic analyses. Surveillance/transmitted drug resistance mutations (SDRMs) were identified according to the 2009 World Health Organization (WHO) list.
A total of 133 (95.7%) samples were successfully amplified and sequenced. Based on phylogenetic analyses of the pol fragment, CRF01_AE accounted for 55.6% (74/133) of the infections, followed by CRF07_BC with 32.3% (43/133), B with 5.3% (7/133), and unique recombinant forms with 6.8% (9/133). A total of 3.0% (4/133) of the subjects were found to harbor HIV variants with SDRMs, including 1.5% with NRTI-related mutations and 1.5% with NNRTI-related mutations. PI-related mutations were absent. The SDRMs included L210W (1.5%), Y181C (0.8%), and G190A (0.8%).
In Anhui, CRF01_AE strains contributed to most of the HIV infections among MSM, and the prevalence of TDR was relatively low in this population. Further studies should be performed to evaluate the trend of TDR among MSM in Anhui and to inform first-line antiretroviral treatment.
Globally, 34.0 million people were living with HIV by the end of 2011. An estimated 0.8% of adults 15–49 years of age worldwide are currently living with HIV-1 . Studies suggest an increasing HIV-1 prevalence among men who have sex with men (MSM), and some evidence indicates that the global prevalence of HIV-1 infection among MSM has increased from 2010 to 2012 [2, 3]. The HIV-1 epidemic in China has also increased. By the end of 2011, there were 780,000 people in China infected with HIV-1, with a national prevalence rate of 0.058%. Of the infected individuals, 63.9% were infected through sexual transmission, including 46.5% through heterosexual transmission and 17.4% through homosexual transmission . Among the estimated 48,000 newly diagnosed cases in 2011, heterosexual transmission accounted for 52.2%, homosexual transmission for 29.4%, and injection drug users (IDUs) for 18.0% of the cases .
Anhui province, located in south-eastern China, neighbors Henan province and was defined as an HIV-1 epidemic focus where most infections were caused by former commercial blood donation . However, newly diagnosed infections have rapidly increased through homosexual transmission among MSM in recent years . To date, 768 MSM have been reported to be infected with HIV in Anhui province.
According to the national guidelines for antiretroviral (ARV) treatment in China, HIV-1-infected persons should receive antiretroviral therapy (ART) when their CD4 counts are less than 350 cells/μL. Extensive use of ARV drugs has led to the increasing transmission of HIV variants with drug resistance mutations that can be detected in individuals before the initiation of treatment [6–9], and gives rise to a reduction in efficacy of first-line ART . From the beginning of national scale-up of combined antiretroviral therapy in China, many HIV drug resistance surveys have been conducted, and viral mutations conferring resistance to protease inhibitors (PIs) and/or reverse transcriptase inhibitors (RTIs) has been found in both ARV-treated patients and treatment-naive patients [11–15]. However, little is known about mutations associated with HIV-1 resistance to PIs and/or RTIs in newly diagnosed ART-naive MSM in Anhui. To provide information about the epidemic trends of HIV and to optimize the treatment strategies in Anhui, the prevalence of surveillance/transmitted drug resistance mutations (SDRMs) was evaluated in ART-naive MSM who were newly diagnosed as HIV-infected in 2011.
Materials and Methods
Study population and specimens
A cross-sectional study was conducted among newly diagnosed HIV-infected MSM in 15 prefectures of Anhui in 2011, according to guidelines of the Anhui Center for Disease Control and Prevention (CDC), which provides HIV/AIDS treatment and prevention services through education, quality services, and compassionate care to those with HIV/AIDS. Our research included the following 15 cities: Fuyang, Bozhou, Huaibei, Huainan, Suzhou, Bengbu, Hefei, Liu’an, Anqing, Huangshan, Xuancheng, Ma’anshan, Wuhu, Tongling and Chizhou. All newly diagnosed MSM with HIV were enrolled, except for MSM with known exposure to ARV drugs. All subjects were included anonymously and provided written informed consent. Demographic data were collected through direct interview using a standardized questionnaire prior to sample donation. Blood samples were collected from 139 individuals. Plasma was isolated within 6 hours of sampling and stored at −80°C until HIV drug resistance genotyping. The study was reviewed and approved by the local Ethical Review Committee of the Anhui CDC.
RT-PCR and nested PCR amplification
A partial pol fragment was amplified and sequenced with an in-house protocol, as previously described [16, 17]. Briefly, viral RNA was extracted from plasma using a QIAamp Viral RNA Mini kit (Qiagen, Valencia, CA, USA). HIV-1 cDNA was obtained and amplified using a one step RT-PCR kit (TaKaRa Biotechnology Co, Dalian, China) with primers RT21 (5’-CTG TAT TTC TGC TAT TAA GTC TTT TGA TGG G-3’, 3509–3539 nt, HBX2) and MAW26 (5’-TTG GAA ATG TGG AAA GGA AGG AC-3’, 2027–2050 nt, HBX2). The RT-PCR product was then used as a template and the nested PCR primers PRO-1 (5’-CAG AGC CAA CAG CCC CAC CA-3’, 2147–2166 nt, HBX2) and RT4R (5’-CTT CTG TAT ATC ATT GAC AGT CCA GCT-3’, 3299–3327 nt, HBX2) were used to amplify a pol fragment that included the whole protease (PR) and partial reverse transcriptase (1–250 amino acids, RT). The PCR products were purified by a gel extraction kit (Qiagen, Valencia, CA) and bidirectly sequenced using BigDye terminator sequencing technology (Applied Biosystems, Foster City, CA, USA) on an Applied Biosystems 3730 Sequencer [18, 19].
The sequences of the pol fragment were aligned with reference sequences from the Los Alamos database using the BioEdit 7.0 software. HIV-1 subtype was determined by phylogenetic analyses using the neighbor-joining method . For specimens of unknown HIV-1 subtype, bootscanning analyses were performed to screen for a recombination breakpoint using Simplot 3.5 software.
Drug resistance genotyping of HIV-1 isolates
The pol sequences were compared with consensus B sequence in the Stanford HIV Drug Resistance Database (HIVdb; http://hivdb.stanford.edu), and surveillance/transmitted HIV-1 drug resistance mutations (SDRMs) were identified according to the 2009 World Health Organization (WHO) list . The levels of drug resistance were analyzed with the HIVdb algorithm.
Median (standard deviation, SD), range and frequencies (%) were used to describe patients’ characteristics. The frequency of SDRMs was also calculated. Statistical analyses were performed with SPSS version 17.0 (SPSS Inc., Chicago, IL, USA).
Characteristics of participants
Characteristics of 139 HIV-1-infected treatment-naive MSM
Married and having spouse
Highest education achieved
College and above
Business service personnel
Sex transmitted diseases
CD4 count: median (SD), range
446 (213), 49–1182
HIV-1 subtypes and recombinant strains
Distribution of HIV-1 subtypes among newly diagnosed MSM (n = 133)
HIV-1 subtypes (n)
Identification and interpretation of surveillance/transmitted drug resistance mutations
Frequency of surveillance/transmitted drug resistance mutations
Genotyping analysis revealed that none of the specimens had SDRMs against PIs. However, some accessory mutations that did not affect susceptibility to ARV drugs were found in the protease gene, including L10I/L/V (3.0%; 4/133), V11I/V (3.0%; 4/133), L33F (2.3%; 3/133), and A71A/V/T (12.8%; 17/133).
Our findings showed that CRF01_AE accounted for the majority (55.6%) of HIV-1-infections in MSM of Anhui province. This is consistent with the results of a national survey of HIV-1 molecular epidemiology in 2006, which reported that CRF07_BC (35.5%), CRF01_AE (27.6%), CRF08_BC (20.1%), and subtype B' (9.6%) were the four predominant HIV-1 strains in China . Furthermore, CRF01_AE has replaced subtype B as the most common subtype among MSM [16, 22]. The dominance of CRF01_AE was observed among MSM in coastal cities and provinces, such as Tianjin (88.9%), Jiangsu (70%), Shandong (77.8%), and Zhejiang (100%), in one province of central China, Shanxi (84.6%), and in one province of western China, Gansu (50%) .
In this study, a high proportion of MSM (30.9%) were married or divorced, raising concerns about the risk of acquiring HIV-1 from or transmitting HIV-1 to their sexual partners. Various forms of HIV transmission, including both homosexual and heterosexual behavior, likely exist among MSM of Liaoning province . Therefore, further study should be carried out to determine which subtypes of HIV-1 predominantly infect the heterosexuals and IDUs.
We focused on HIV-1-infected treatment-naive MSM in Anhui province, and found that the overall TDR rate was 3.0%, slightly lower than the national level and Liaoning province [23, 24]. The first nationwide survey of TDR in HIV-1-infected treatment-naive individuals was conducted in 2005, and the national TDR rate was reported to be 3.8% . In 2011, the overall TDR rate was 4.9% among HIV-1-infected treatment-naive MSM in 19 Chinese provinces/cities (Anhui province was not included) . According to an investigation in Liaoning province, 4.5% of ART-naive MSM had drug-resistant strains .
We report that 1.5% of samples were resistant to NRTIs, 1.5% of samples were resistant to NNRTIs, and none were resistant to PIs. Specific mutations included L210W, Y181C, and G190A, which confers different levels of drugs resistance. However, we detected many minor mutations which could compensate for the reduced fitness of resistant mutants, even though the minor mutations have not previously been associated with decreased in vitro susceptibility. Other commonly observed mutations were T69A/N/S, V179E, L10I/L/V, V11I/V, L33F, and A71A/T/V; none of these mutations is known to confer drug resistance to NRTIs, NNRTIs, or PIs.
Specific polymorphisms may occur in particular subtypes. For example, L10V, V11I/V, L33F, T69A/N/S, and P236L were present only in individuals with CRF01_AE strains. These mutations do not affect the susceptibility to ARV drugs. However, they might influence the emergence of drug-resistant viruses by modifying drug susceptibility and/or virus replication . The present study found that substitutions of A71T/V and L10I often appeared in CRF07_BC, which was different from other studies reporting that substitutions of A71T/V often appeared in B viruses, whereas L10I was frequently discovered in CRF01_AE viruses . A98G and V179E were excluded from the WHO list of SDRMs because of high occurrence in viruses from treatment-naive individuals. A98G has been shown to confer low-level resistance against EFV and NVP, and V179D in CRF01_AE/CRF07_BC recombinant viruses has been shown to confer low-level resistance against EFV and intermediate level resistance against NVP, while V179D in CRF01_AE viruses does not affect resistance to any RT drugs (data not published). This phenomenon is worthy of further study.
Our data represent only TDR in HIV-1-infected treatment-naive MSM in Anhui province; therefore, the results may not be representative of other areas. Nevertheless, these data warn us that HIV transmitted-drug-resistant isolates may be prevalent among HIV-1-infected treatment-naive MSM, which will no doubt compromise the success of future ART in these patients.
There were several limitations in our study. First, although all the subjects were newly diagnosed as HIV-infected, the date of their infections was not known. However, it is suggested that a substantial proportion of the subjects were infected recently, because more than half of the individuals had CD4 counts of ≥350 cells/μl, and almost half were between 18–29 years old. Second, the prevalence of TDR might be underestimated, because some mutations may have disappeared over time due to lack of replicative fitness. However, some NNRTI-related mutations, such as K103N, can persist for a long period of time [27, 28].
This study is the first to describe HIV diversity and TDR prevalence in ART-naive, HIV-1-infected MSM in Anhui province. CRF01_AE was found to be a major subtype, TDR prevalence was relatively low, and most TDR cases were associated with RTIs. These findings provide baseline information of HIV-1 transmitted-drug-resistant mutations, which has implications for HIV/AIDS prevention and treatment programs among MSM patients in China. Further studies of TDR are necessary to determine the trend of TDR in Anhui province and to optimize treatment regimens in the future.
This work was supported by the Anhui Provincial Program of Preventive Medicine & Public Health (Grant#2011Y0102) and the National Grand Program on Key Infectious Disease Control (Grant#2012ZX10001-002-001-002). The authors thank all patients for their participation in this study and all those who have been involved in surveillance, laboratory testing, and treatment for HIV in Anhui province, China. We thank Dr. Lingjie Liao at the National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention for her suggestions, revising, and language editing. We also thank Furong Li at Anhui Provincial Center for Disease Control and Prevention for her language editing.
- UNAIDS: 2012 UNAIDS Report on the Global AIDS Epidemic. [Available at http://www.unaids.org/en/resources/publications/2012/Google Scholar
- Beyrer C, Baral SD, van Griensven F, Goodreau SM, Chariyalertsak S, Wirtz AL, Brookmeyer R: Global epidemiology of HIV infection in men who have sex with men. Lancet. 2012, 380: 367-377.PubMed CentralView ArticlePubMedGoogle Scholar
- van Griensven F, de Lind van Wijngaarden JW: A review of the epidemiology of HIV infection and prevention responses among MSM in Asia. AIDS. 2010, 24 (Suppl 3): S30-S40.http://dx.doi.org/10.1097/01.aids.0000390087.22565.b4, View ArticlePubMedGoogle Scholar
- Ministry of Health, People's Republic of China, Joint United Nations Programme on HIV/AIDS World Health Organization : 2011 Estimates for the HIV/AIDS epidemic in China. Chin J AIDS STD. 2012, 18: 1-5. In ChineseGoogle Scholar
- Xu JQ, Wang JJ, Han LF, Xu C, Ruan YH, Xu ZH, Chen X, Liu ZD, Wang J, Su B, Ding XP, Gao B, Gu YB, Cao XY, Xing H, Hong KX, Peng H, Zhao QB, Yuan L, Feng Y, Zhang GY, Ma LY, Wu L, Shao YM: Epidemiology, clinical and laboratory characteristics of currently alive HlV-1 infected former blood donors naïve to antiretroviral therapy in Anhui Province, China. Chin Med J (Engl). 2006, 119: 1941-1948.Google Scholar
- Cardoso LP, Queiroz BB, Stefani MM: HIV-1 pol phylogenetic diversity and antiretroviral resistance mutations in treatment naïve patients from Central West Brazil. J Clin Virol. 2009, 46: 134-139.http://dx.doi.org/10.1016/j.jcv.2009.07.009, View ArticlePubMedGoogle Scholar
- Geretti AM: Epidemiology of antiretroviral drug resistance in drug-naive persons. Curr Opin Infect Dis. 2007, 20: 22-32.View ArticlePubMedGoogle Scholar
- Hattori J, Shiino T, Gatanaga H, Yoshida S, Watanabe D, Minami R, Sadamasu K, Kondo M, Mori H, Ueda M, Tateyama M, Ueda A, Kato S, Ito T, Oie M, Takata N, Hayashida T, Nagashima M, Matsuda M, Ibe S, Ota Y, Sasaki S, Ishigatsubo Y, Tanabe Y, Koga I, Kojima Y, Yamamoto M, Fujita J, Yokomaku Y, Koike T: Trends in transmitted drug-resistant HIV-1 and demographic characteristics of newly diagnosed patients: nationwide surveillance from 2003 to 2008 in Japan. Antiviral Res. 2010, 88: 72-79.http://dx.doi.org/10.1016/j.antiviral.2010.07.008, View ArticlePubMedGoogle Scholar
- Wheeler WH, Ziebell RA, Zabina H, Pieniazek D, Prejean J, Bodnar UR, Mahle KC, Heneine W, Johnson JA, Hall HI, : Prevalence of transmitted drug resistance associated mutations and HIV-1 subtypes in new HIV-1 diagnoses, U.S.-2006. AIDS. 2010, 24: 1203-1212.http://dx.doi.org/10.1097/QAD.0b013e3283388742, View ArticlePubMedGoogle Scholar
- Wittkop L, Gunthard HF, de Wolf F, Dunn D, Cozzi-Lepri A, de Luca A, Kücherer C, Obel N, von Wyl V, Masquelier B, Stephan C, Torti C, Antinori A, García F, Judd A, Porter K, Thiébaut R, Castro H, van Sighem AI, Colin C, Kjaer J, Lundgren JD, Paredes R, Pozniak A, Clotet B, Phillips A, Pillay D, Chêne G, : Effect of transmitted drug resistance on virological and immunological response to initial combination antiretroviral therapy for HIV (EuroCoordCHAIN joint project): a European multicohort study. Lancet Infect Dis. 2011, 11: 363-371.http://dx.doi.org/10.1016/S1473-3099(11)70032-9, View ArticlePubMedGoogle Scholar
- Han XX, Zhang M, Cui WG, Liu BG, Wang Y, Zhang ZN, Liu J, Geng WQ, Diao YY, Dai D, Jiang YJ, Shang H: Efficacy of anti-HIV treatment and drug-resistance mutations in some parts of China. Zhonghua Yi Xue Za Zhi. 2005, 85: 760-764. In ChinesePubMedGoogle Scholar
- Li JY, Li HP, Li L, Wang Z, Yang K, Bao ZY, Zhuang DM, Liu SY, Liu YJ, Xing H, Shao YM: Prevalence and evolution of drug resistance HIV-1 variants in Henan, China. Cell Res. 2005, 15: 843-849.View ArticlePubMedGoogle Scholar
- Yang K, Li JY, Bao ZY, Li HP, Li L, Zhuang DM, Wang Z, Li H: Genotypic antiretroviral resistance testing and phylogenetic analysis of protease and reverse transcriptase in antiretroviral drug-naive AIDS patients in Henan province. Zhonghua Liu Xing Bing Xue Za Zhi. 2005, 26: 351-355. In ChinesePubMedGoogle Scholar
- Wu J, Xing H, Shen Y, Liao L, Su B, Shao Y: Study on transmission of drug resistant human immunodeficiency virus I naive infection in Anhui province in 2008. Chin J Control Prev. 2011, 15: 33-35. In ChineseGoogle Scholar
- Shen Y, Su B, Wu J, Liu A, Cheng X: An analysis of patterns of HIV drug resistance in case of failure of first-line anti-retroviral therapy. Chin J AIDS STD. 2011, 17: 295-301. In ChineseGoogle Scholar
- Zhang X, Li S, Li X, Li X, Xu J, Li D, Ruan Y, Xing H, Zhang X, Shao Y: Characterization of HIV-1 subtypes and viral antiretroviral drug resistance in men who have sex with men in Beijing, China. AIDS. 2007, 21 (Suppl 8): S59-S65.http://dx.doi.org/10.1097/01.aids.0000304698.47261.b1, View ArticlePubMedGoogle Scholar
- Liao L, Xing H, Li X, Ruan Y, Zhang Y, Qin G, Shao Y: Genotypic analysis of the protease and reverse transcriptase of HIV type 1 isolates from recently infected injecting drug users in western China. AIDS Res Hum Retroviruses. 2007, 23: 1062-1065.View ArticlePubMedGoogle Scholar
- Wang W, Meng Z, Zhou M, Guo C, Zhang F, Xu J, Zhang X: Near full-length sequence analysis of two new HIV type 1 unique (CRF01_AE/B) recombinant forms among men who have sex with men in China. AIDS Res Hum Retroviruses. 2012, 28: 411-417.http://dx.doi.org/10.1089/AID.2011.0196, View ArticlePubMedGoogle Scholar
- Meng Z, Xing H, He X, Ma L, Xu W, Shao Y: Genetic characterization of three newly isolated CRF07_BC near full-length genomes in China. AIDS Res Hum Retroviruses. 2007, 23: 1049-1054.View ArticlePubMedGoogle Scholar
- Wu J, Meng Z, Xu J, Lei Y, Jin L, Zhong P, Han R, Su B: New emerging recombinant HIV-1 strains and close transmission linkage of HIV-1 strains in the Chinese MSM population indicate a new epidemic risk. PLoS One. 2013, 8: e54322-http://dx.doi.org/10.1371/journal.pone.0054322, PubMed CentralView ArticlePubMedGoogle Scholar
- Bennett DE, Camacho RJ, Otelea D, Kuritzkes DR, Fleury H, Kiuchi M, Heneine W, Kantor R, Jordan MR, Schapiro JM, Vandamme AM, Sandstrom P, Boucher CA, van de Vijver D, Rhee SY, Liu TF, Pillay D, Shafer RW: Drug resistance mutations for surveillance of transmitted HIV-1 drug-resistance: 2009 update. PLoS One. 2009, 4: e4724-http://dx.doi.org/10.1371/journal.pone.0004724, PubMed CentralView ArticlePubMedGoogle Scholar
- He X, Xing H, Ruan Y, Hong K, Cheng C, Hu Y, Xin R, Wei J, Feng Y, Hsi JH, Takebe Y, Shao Y, : A comprehensive mapping of HIV-1 genotypes in various risk groups and regions across China based on a nationwide molecular epidemiologic survey. PLoS One. 2012, 7: e47289-http://dx.doi.org/10.1371/journal.pone.0047289, PubMed CentralView ArticlePubMedGoogle Scholar
- Yang J, Xing H, Niu J, Liao L, Ruan Y, He X, Feng Y, Li Z, Shao Y: The emergence of HIV-1 primary drug resistance genotypes among treatment-naïve men who have sex with men in high-prevalence areas in China. Arch Virol. 2013, 158: 839-844.http://dx.doi.org/10.1007/s00705-012-1557-7, View ArticlePubMedGoogle Scholar
- Zhao B, Han X, Dai D, Liu J, Ding H, Xu J, Chu Z, Bice T, Diao Y, Shang H: New trends of primary drug resistance among HIV type 1-infected men who have sex with men in Liaoning Province, China. AIDS Res Hum Retroviruses. 2011, 27: 1047-1053.http://dx.doi.org/10.1089/AID.2010.0119, View ArticlePubMedGoogle Scholar
- Holguín A, de Arellano Ramirez E, Rivas P, Soriano V: Efficacy of antiretroviral therapy in individuals infected with HIV-1 non-B subtypes. AIDS Rev. 2006, 8: 98-107.PubMedGoogle Scholar
- Liu J, Yue J, Wu S, Yan Y: Polymorphisms and drug resistance analysis of HIV-1 CRF01_AE strains circulating in Fujian Province, China. Arch Virol. 2007, 152: 1799-1805.View ArticlePubMedGoogle Scholar
- Polilli E, Parruti G, Cosentino L, Sozio F, Saracino A, Consorte A, Angarano G, Di Masi F, Mazzotta E, Fazii P: Rapid and persistent selection of the K103N mutation as a majority quasispecies in a HIV1-patient exposed to efavirenz for three weeks: a case report and review of the literature. J Med Case Rep. 2009, 3: 9132-http://dx.doi.org/10.4076/1752-1947-3-9132, PubMed CentralView ArticlePubMedGoogle Scholar
- Drescher SM, von Wyl V, Yang WL, Böni J, Yerly S, Shah C, Aubert V, Klimkait T, Taffé P, Furrer H, Battegay M, Ambrosioni J, Cavassini M, Bernasconi E, Vernazza PL, Ledergerber B, Günthard HF, Kouyos RD, : Treatment-naive individuals are the major source of transmitted HIV-1 drug resistance in men who have sex with men in the Swiss HIV Cohort Study. Clin Infect Dis. 2014, 58: 285-294.http://dx.doi.org/10.1093/cid/cit694, View ArticlePubMedGoogle Scholar
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