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Fig. 1 | AIDS Research and Therapy

Fig. 1

From: Duplicate analysis method: a cheaper alternative to commercial IQC materials in limited resource settings for monitoring CD4 testing

Fig. 1

Representative displays of fresh and stabilized blood samples on FACSCount, FACSCalibur and Cyflow. Equipment displays in row 1 are for fresh blood sample used in duplicate analysis method, row 2 are for the multi-check stabilized blood sample and row 3 are for IMMUNO-TROL controls. a, d, g are from FASCount; b, e, h from FACSCalibur and c, f, i from Cyflow. BD FACSCount CD4/CD3 reagent kit contained anti CD3 PE-Cy5 and anti CD4 PE antibodies. Tricolour reagent used for FACSCalibur analysis contained anti CD45 PerCP, anti CD3 FITC and anti CD4 PE antibodies. The gates shown in the figure are autogates set by the Multiset software used for FACSCalibur analysis. CD4 easy count kit used for Cyflow contained anti CD4 PE antibody for staining, where the gates were set manually.

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AIDS Research and Therapy

ISSN: 1742-6405

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