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Fig. 6 | AIDS Research and Therapy

Fig. 6

From: Defining the fitness of HIV-1 isolates with dual/mixed co-receptor usage

Fig. 6

Fitness of the dual tropic env chimeric virus when blocking the virus component using CCR5 or CXCR4. a The dm env chimeric viruses (B12 and B19) were competed against the x4 reference viruses (all titered on U87.CD4.CXCR4 cells) in U87.CD4.CXCR4 cultures or in PHA-stimulated, IL-2 treated PBMC cultures with or without maraviroc. The fitness differences are plotted as described in Fig. 2 and “Methods”. b The level of maraviroc inhibition of the monoinfections or dual infections was measured by relative RT activity in the cell free supernatant and plotted as a percentage of the no drug control. c The env chimeric HIV-1 and r5 reference strains (B2 and C3) were titered on U87.CD4.CCR5 cells and then competed together in PHA-stimulated, IL-2 treated PBMC cultures with or without AMD3100. The same dual infection with the same MOI (0.004) was repeated in U87.CD4.CCR5 cultures. The fitness differences are plotted as described in Fig. 2 and “Methods”. d The level of AMD3100 inhibition of the monoinfections or dual infections was measured by relative RT activity in the cell free supernatant and plotted as a percentage of the no drug control. All experiments were performed in triplicate with the exception of c, d (performed in duplicate) due to limited supply Of AMD3100.

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