Specific killing of HIV-1 infected cells. (a) H9 lymphocyte T cells were infected by the WT HIV-1 at MOI of 0.1, exactly as described in , and then the infected cells were treated every two days with the indicated molecules or combinations. Every two days a sample was removed and its virus titer was estimated by the MAGI assay  using TZM-bl cells exactly as described . (b) H9 lymphocyte T cells were infected with WT HIV-1 at the indicated MOIs and treated with INS or INS+INrs. The amount of virus production was estimated using MAGI assay on TZM-bl cells at 48 h PI. (c) Same as (a) but cells viability was estimated by the MTT assay as described in . (d
) and (d
) Same as (a) but the average amount of viral cDNA integration events/cells was estimated by quantitative hemi-nested Real Time PCR exactly as described in . Cells were grown as described in . Viruses were produced and viral stock titer was estimated as described in . Peptides were synthesized and purified as described in [28, 31]. The following concentrations were used: AZT 2 μM, Ro 31-8959 10 nM, INS/INrs 150 μM. Every experiment was preformed at least three times with relative error not more ±10%. Error bars represent standard deviation.