Figure 5

Virus particle production in Jurkat T-cells transduced with different HIV-1 vectors upon challenge with a replication defective HIV-1. Jurkat T-cells were separately transduced with each of the indicated vectors (X-axis) and sorted to greater than 95% purity. Each population was either mock-infected or infected with VSV-G pseudotyped pNL4-3.HSA. R^-E^-. The supernatants from mock or virus-infected cells were obtained on days 1, 4 and 7 and assayed for HIV-1 p24 capsid protein using a commercial ELISA kit. The Y-axis shows mean p24 levels produced by each of the different cell populations on days 4 and 7 normalized to the p24 produced by infected but untransduced Jurkat cells (which was set at 100%). The results shown are from three independent experiments. Error bar = 1 SD.