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Figure 1 | AIDS Research and Therapy

Figure 1

From: Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes

Figure 1

Schematic representation of HIV-1 packaging and gene transfer vector constructs containing HIV-1 or SIV RRE. A) The packaging constructs contain Gag/Pol coding sequence derived from pNL4-3 inserted downstream of the human cytomegalovirus (CMV) immediate early promoter in pCDNA3. The RRE from HIV-1 (350 nt) or from SIVmac239 (272 or 1045 nt) was positioned downstream of the Gag/Pol coding sequence. Polyadenylyation sequence in pCDNA3 is derived from bovine growth hormone gene (BGHpA). B) The gene transfer vectors were derived from pNL4-3 and contain a transgene expression cassette consisting of Elongation factor 1 alpha promoter/enhancer elements (EF1α) driving the enhanced green fluorescent protein (EGFP) or a fusion protein consisting of EGFP-2A-Rev M10. Woodchuck post-transcriptional regulatory element (WPRE) was positioned downstream of the transgene. HIV-1 or SIV RRE was present upstream of the transgene expression cassette. Δψ: Deletion in the HIV-1 encapsidation signal between nt 751 and nt 779 of pNL4-3; LTR: HIV-1 long terminal repeat; FS: Frame-shift mutation in gag; CPPT/CTS: Central polypurine tract/central termination sequence; 2A: Foot and mouth disease virus 2A cleavage factor; M10: Rev M10; 5'ss: 5' splice site; 3'ss: 3' splice site.

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AIDS Research and Therapy

ISSN: 1742-6405

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