Evaluation of C14 capability to limit the infectiousness of viral particles. HIV-1 was adsorbed on poly-L-lysin pre-coated wells (Greiner Bio-One) at 4°C overnight and further incubated with C14 for 1 h. In positive and negative control wells, 1% Triton X-100 and medium were added, respectively. After four washes, activated peripheral blood lymphocytes were incubated with C14 or triton-treated or untreated HIV-1 particles. After 6 days, viral production was assessed by p24 Ag capture ELISA. Means are representative of 3 independent experiments were performed in triplicates. **, p ≤ 0.01.