Evaluation of C14 toxicity on primary cells. Monocyte-derived macrophages, monocyte-derived dendritic cells and T cells were cultured with increased concentrations of C14 (ranged from 1 to 12 μM) for 24 hours. After washing, culture viability was determined by using the MTT-cytotoxicity assay according to the manufacturer's instructions. The values given are the mean viability ± 1 standard deviation of primary cells, expressed in percentage. Means are representative of 3 independent experiments and assays were performed in duplicates.