Figure 1
From: Bacterial vaginosis and human immunodeficiency virus infection
Bacterial Vaginosis induces IL-12p70 production by Dendritic Cells. Monocyte-derived dendritic cells (MDDC) were produced from monocytes isolated from the blood of normal donors using standard methods [46]. MDDC were incubated for 48 hours with either culture medium alone (Medium), lipopolysaccharide at 1 μg/ml (LPS), or genital tract secretions collected by cervicalvaginal lavage from women with BV (BV CVL) or normal flora (Normal CVL). The BV CVL and Normal CVL were pools of equal amounts of CVL from 15 and 14 women respectively. Status of CVL donors was determined by gram stain. Supernatants were harvested and analyzed for IL-12p70 by ELISA.