MMP Vector | Phoenix (Amphotropic) | Phoenix (GaLV) |
---|
 | Percent GFP1 | Proviral copies2 | Titer (TU/ml)3 | Percent GFP1 | Proviral copies2 | Titer (TU/ml)3 |
Empty | 7% | 0.82 | 1.0 × 105 | 68% | 29 | 2.6 × 105 |
70.15 | 18% | 0.33 | 1.4 × 105 | 69% | 26 | 2.5 × 105 |
70.8 | 17% | 0.80 | 1.1 × 105 | 59% | 15 | 2.1 × 105 |
80.55 | 14% | 0.97 | 1.2 × 105 | 64% | 33 | 2.5 × 105 |
- 1Initial percentage of GFP+ cells in each packaging cell line after transduction and prior to cell sorting.
- 2Molecular analysis of number of proviral copies of the GFP gene in each packaging cell line as determined by real-time PCR of genomic DNA.
- 3Calculated titer of the supernatant from each packaging cell line after limiting dilution and analysis of the expression of GFP in U2OS cells.