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Table 1 Characterization of Phoenix (Amphotropic) and Phoenix (GaLV) packaging cell lines.

From: Instability of retroviral vectors with HIV-1-specific RT aptamers due to cryptic splice sites in the U6 promoter

MMP Vector

Phoenix (Amphotropic)

Phoenix (GaLV)

 

Percent GFP1

Proviral copies2

Titer (TU/ml)3

Percent GFP1

Proviral copies2

Titer (TU/ml)3

Empty

7%

0.82

1.0 × 105

68%

29

2.6 × 105

70.15

18%

0.33

1.4 × 105

69%

26

2.5 × 105

70.8

17%

0.80

1.1 × 105

59%

15

2.1 × 105

80.55

14%

0.97

1.2 × 105

64%

33

2.5 × 105

  1. 1Initial percentage of GFP+ cells in each packaging cell line after transduction and prior to cell sorting.
  2. 2Molecular analysis of number of proviral copies of the GFP gene in each packaging cell line as determined by real-time PCR of genomic DNA.
  3. 3Calculated titer of the supernatant from each packaging cell line after limiting dilution and analysis of the expression of GFP in U2OS cells.
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AIDS Research and Therapy

ISSN: 1742-6405

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