Figure 4

IL-1, TNF-α, IL-6 and IFN-γ assays in blood culture supernatants: Blood cultures from HIV patients were treated as follows, no treatment, infection with H37Rv, and infection with H37Rv and treatment with NAC. Cultures were terminated at 48 h, after infection. Uninfected cultures were terminated at the same time points. Cultures were centrifuged at 2000 rpm for 10 min. Cell free supernatants were used for assay of IL-1 (Fig 4a), TNF-α (Fig 4b), IL- 6 (Fig 4c) and IFN-γ (Fig 4d). Cytokine assay was performed using a Beadlyte kit procured from Upstate. The assay was performed as per manufacturer's protocol.