Figure 4

CD34+ progenitor cell derived monocytes stably expressing U16RBE are resistant to HIV-1 infection. CD34+ cells isolated from human cord blood were first transduced with either the parental MND/eGFP vector or the MND/eGFP U16RBE and then sorted for eGFP expression. (A) total RNA isolated from the eGFP sorted, transduced CD34+ cells was electrophoresed in a 6% polyacrylamide-7M Urea gel and blotted onto a nylon filter. Hybridizations were performed using a probe complementary to the 3' end of the U16 snoRNA and a probe specific for the tRNA₃^Lys used as a loading control. (B) Parental CD34+ cells along with the transduced and eGFP sorted CD34+ cells were challenged with HIV-1 JFRL using an m.o.i. of 0.01. On days 1, 7, 14, 21 and 28 days post-infection the accumulation of the viral p24^gag antigen was assayed. The data presented represent an average of two independent challenge experiments, and the standard errors for each point are approximately 5%