Figure 4

Reactivities of serum IgG from HIV-1-infected subjects with HIV-1 gp120 produced in different cell lines analyzed by Western blot (A, C) and dot-blot (B, D). HIV-1 Env gp120 glycoforms produced in HEK 293T, Jurkat, RD, HepG2 and CHO cells were (A) separated by SDS-PAGE under reducing conditions and blotted onto a PVDF membrane or (B) dot blotted onto a PVDF membrane and both were developed with sera (0.2 μg/ml gp120-specific IgG) from HIV-1-infected subjects (P1-9; HIV-1+ sera) or sera (1:200 dilution) from healthy sero-negative subjects (C1-3; Control sera). Results from one of two experiments are shown. (C, D) Densitometric analysis performed with the ImageJ program shows mean reactivity of each serum IgG with individual gp120 variants.