A possible role for CCR5 in the progression of atherosclerosis in HIV-infected patients: a cross-sectional study

Table 1 Summary of taqman® gene expression assays used in this study*

Gene symbol	Assay ID*	Context sequence	Amplicon length (bp)
CX3CR1	Hs00365842_m1	CAGTCCACGCCAGGCCTTCACCATG	84
CCR2	Hs00174150_m1	GGGGAGAAGTTCAGAAGCCTTTTTC	74
CXCR4	Hs00607978_s1	CCTGTCCTGCTATTGCATTATCATC	153
CCR5	Hs00152917_m1	GAAACTCTCCCCGGGTGGAACAAGA	98
GAPDH	Hs99999905_m1	TTGGGCGCCTGGTCACCAGGGCTGC	124

*Thermal cycling and fluorescence detection was performed on the Applied Biosystems ABI Prism 7900HT Detection System with ABI Prism 7900HT SDS Software 2.2. Forty cycles were run with the following parameters: 2 mins at 50°C and 10 mins at 94.5°C and for each cycle 30 secs at 97°C for denaturation and 1 min at 59.7°C for transcription. FAM was the reporter dye.

ISSN: 1742-6405