Figure 4

RT-PCR detection of Tar RNA in differentiated macrophages: Total cellular RNA was extracted from control and transgenic macrophages and subjected to RT-PCR using primers specific for HIV-1 Tar decoy. A. HIV-1 Tar specific amplification (125 bp). 1, control macrophages; 2, HIV-1-Tar vector transduced macrophages; 3, HIV-1-Tar-CCR5Rz vector transduced macrophages. B. β-actin RNA amplified in corresponding lanes as controls.