Table 2 Operational comparison of the two methodologies
Sr. No. | Parameter | EasyCD4 System | Conventional flow cytometry (FACSort) |
|---|---|---|---|
1. | Ease of performance | Excellent | Good |
2. | Volume of blood required/test | 10 μl | 100 μl |
3. | Reagents required/test | ||
a. Antibodies | 1 μl/sample | 20 μl/sample | |
b. Sheath fluid | --- | 50 ml/sample | |
c. RBC Lysing solution | 18 μl/sample | 120 μl/sample | |
4. | Generation of waste/test | 200 μl/sample | 18 ml/sample |
5. | Time required to process one sample | 35 minutes | 90 minutes |
6. | Routine maintenance: Cleaning procedure | Daily (5 minutes) Monthly (-----) | Daily (20 minutes) Monthly (90 minutes) |