Figure 1

Aβ(1–42) fibrils but not mono- and oligomers enhance HIV-1 infection of TZM-bl cells. (A) Equal amounts (500 TCID₅₀ as determined with TZM-bl cells using supernatant of transfected HEK 293T cells) of the dual-tropic HIV-1 lab strain NL4-3 PI 952 [11] were pre-incubated for 5 min at RT with Aβ(1–42) fibrils. Subsequently, the pretreated viruses were used to infect TZM-bl reporter cells and infection-induced luciferase activity was assayed 48 h post infection. (*** p < 0.001, * p < 0.05 referred to PBS treated and infected cells). (B) X-fold change of luciferase enhancement was quantified relative to cells infected in the absence of Aβ(1–42) fibrils (PBS). (C) Luciferase RLUs of non-infected cells, which were treated with the indicated concentrations of Aβ(1–42). (D) Chromatogram of a size exclusion chromatography (SEC) showing the absorption profile of Aβ(1–42) monomers (M) and oligomers (O), which were used in the following analysis. (E and F) The same experiments as in (A) but viruses were pre-incubated with Aβ(1–42) mono- and oligomers obtained by SEC shown in (D). milli-absorbance-units (mAu); non-infected (n.i.); relative light units (RLU); size exclusion chromatography buffer (SB).