Daily variations of gut microbial translocation markers in ART-treated HIV-infected people.

BACKGROUND
Increased intestinal barrier permeability and subsequent gut microbial translocation are significant contributors to inflammatory non-AIDS comorbidities in people living with HIV (PLWH). Evidence in animal models have shown that markers of intestinal permeability and microbial translocation vary over the course of the day and are affected by food intake and circadian rhythms. However, daily variations of these markers are not characterized yet in PLWH. Herein, we assessed the variation of these markers over 24 h in PLWH receiving antiretroviral therapy (ART) in a well-controlled environment.


METHODS
As in Canada, PLWH are predominantly men and the majority of them are now over 50 years old, we selected 11 men over 50 receiving ART with undetectable viremia for more than 3 years in this pilot study. Blood samples were collected every 4 h over 24 h before snacks/meals from 8:00 in the morning to 8:00 the next day. All participants consumed similar meals at set times, and had a comparable amount of sleep, physical exercise and light exposure. Plasma levels of bacterial lipopolysaccharide (LPS) and fungal (1→3)-β-D-Glucan (BDG) translocation markers, along with markers of intestinal damage fatty acid binding protein (I-FABP) and regenerating islet-derived protein-3α (REG3α) were assessed by ELISA or the fungitell assay.


RESULTS
Participants had a median age of 57 years old (range 50 to 63). Plasma levels of BDG and REG3α did not vary significantly over the course of the study. In contrast, a significant increase of LPS was detected between 12:00 and 16:00 (Z-score: - 1.15 ± 0.18 vs 0.16 ± 0.15, p = 0.02), and between 12:00 and 24:00 (- 1.15 ± 0.18 vs 0.89 ± 0.26, p < 0.001). The plasma levels of I-FABP at 16:00 (- 0.92 ± 0.09) were also significantly lower, compared to 8:00 the first day (0.48 ± 0.26, p = 0.002), 4:00 (0.73 ± 0.27, p < 0.001) or 8:00 on secondary day (0.88 ± 0.27, p < 0.001).


CONCLUSIONS
Conversely to the fungal translocation marker BDG and the gut damage marker REG3α, time of blood collection matters for the proper evaluation for LPS and I-FABP as markers for the risk of inflammatory non-AIDS co-morbidities. These insights are instrumental for orienting clinical investigations in PLWH.


Background
The gastrointestinal tract is a distinctive tissue with physical, biological and immunological barriers, allowing nutrient absorption while preventing the translocation of microbes and their products. HIV infection is associated with modification of the gut microbiota, disruption of the gut epithelial barrier, and increased intestinal permeability [1][2][3][4]. In contrast to the global health improvement occurring in people living with HIV (PLWH) receiving antiretroviral therapy (ART), gut damage persists and translocation of microbial products from the gut lumen into the circulation contributes to inflammatory non-AIDS comorbidities [5]. Microbial translocation is one of the main drivers for the development of such comorbidities including cardiovascular disease, depression and cancer in ARTtreated PLWH [6][7][8][9][10].
Circulating intestinal fatty acid binding protein (I-FABP) and regenerating islet-derived protein-3α (REG3α) are two validated gut damage markers in PLWH [40,41]. I-FABP, an intracellular protein constitutively expressed in enterocytes, is released upon cell death and subsequently detected in the blood in inflammatory bowel diseases (IBD) and HIV infection [42,43]. REG3α, an antimicrobial peptide secreted by intestinal Paneth cells into the gut lumen and upon gut damage, translocates into the blood [41]. We observed that REG3α plasma levels were correlated with HIV disease progression, microbial translocation and immune activation in PLWH [41].
As levels of gut damage and microbial translocation markers are low in healthy people and significantly elevated in PLWH [44,45], knowing their daily variations could improve clinical care and research. Herein, we assessed the variation of the microbial translocation markers, LPS and BDG, and the gut damage markers, I-FABP and REG3α, over the course of 24 h in ARTtreated PLWH in a well-controlled environment.

Participants and study design
In this pilot study, 11 men were recruited as they represent the population most affected by HIV in Canada. Inclusion criteria included men over the age of 50, receiving ART with undetectable viremia for more than 3 years. Participants with opportunistic infections (including fungal infections), co-infection with hepatitis B or C, chronic colitis or any other acute conditions were excluded. A total of 11 participants were enrolled and hospitalized for 40 h at the 12-bed phase I clinic of the Centre Hospitalier de l'Université de Montréal, Montréal, QC, Canada. Study timeline is shown in Fig. 1. Blood samples were collected using a catheter fixed to the median cubital vein throughout their hospitalization to prevent repeated venipuncture and disturbing participants' sleep cycles. All the participants were admitted to hospital before 17:00 of the previous day in order for all participants to have the same meal and similar amount of sleep before baseline sampling. Samples were first collected at 8:00 am, and then every 4 h until 8:00 am the next morning for a total of seven timepoints. Plasma was isolated from whole blood and frozen at − 80 °C. All participants had similar meals/snacks at set times (8:30, 13:00, 16:30 and 20:30), and had a comparable amount of sleep, physical exercise and light exposure. All participants were offered the same meals, with a balance of carbohydrates, proteins, fats and Keywords: Daily variation, Microbial translocation, (1 → 3)-β-D-Glucan, Gut damage, HIV fibers. Meals did not contain mushrooms nor seaweed. BDG containing foods such as oat bran were not offered to participants. Scientific and artistic educational presentations were organized as part of a knowledge transfer and exchange with participants and research nurses. All participants agreed to take part in a 60 min low-intensity yoga session in the afternoon. Neither alcoholic beverages nor recreational drugs were permitted during the time of hospitalization.

Statistical analyses
Statistical analyses were conducted using GraphPad Prism 6.0 (La Jolla, CA, USA). In order to analyze the time variation of parameters while accounting for inter-participant variability, the individual raw data were converted to Z scores as previously described [46]. Z-scores were calculated by normalizing each datapoint to the mean of the seven timepoints and standard deviation (SD) of the individual according to the formula: (datapoint-mean)/SD.
Comparisons were conducted between timepoints using non-parametric Kruskal-Wallis test with Dunn's post hoc test. An α-level of 5% was used for statistical significance.

Study participant characteristics
Participant characteristics were summarized in Table 1. All participants were male, with a median age of 57 years (range 50 to 63). Participants received ART for a median of 17 years (range 13 to 22). Plasma VL of all participants were below the level of detection (< 20 copies/ml). Median CD4 T-cell count was 606 (311-1082) cells/µl and CD8 T-cell count was 613 (331-1425) cells/µl.
Conversely, levels of BDG did not vary significantly over the course of the study (Fig. 2d-f, p = 0.261).

Daily variation of the gut damage markers I-FABP and REG3α
Over the course of the study, I-FABP levels varied significantly (p < 0.001). I-FABP levels decreased from 8:00  (Fig. 2g).
Levels of the gut damage marker REG3α did not vary significantly over the course of the study (Fig. 2J-l, p = 0.570).

Discussion
We observed a daily variation of plasma I-FABP with a median difference of 2060 pg/ml between the highest and lowest values, representing a 4.2-fold intraday variation. The median plasma LPS range between highest and lowest values was 18 pg/mL, representing a 1.6fold intraday change. Such variations might explain the breadth of I-FABP and LPS levels observed in other ARTtreated PLWH studies where time of sampling was not documented or considered [13,28,41]. Previous work in mouse models has shown a postprandial increase in LPS levels [47]. We observed a clinically relevant decrease of LPS after breakfast and an increase after dinner which might be explained by natural variations in circadian rhythm [48,49]. These results suggest that sampling LPS from fasting PLWH might decrease variation throughout the day. Similarly, I-FABP was subject to daily variations with the lowest level at 16:00 and highest at 4:00-8:00. However, plasma levels of BDG and REG3α showed no significant variation and were not affected by food intake, time of sampling, or day/night shifts. These findings further validate the use of BDG and REG3α as markers of microbial translocation and gut damage, respectively in ART-treated PLWH.
Translocation of bacterial and fungal products is driven by epithelial gut damage and depletion of intestinal CD4-T cells and contributes to immune activation in HIV [35,50]. Clinical studies commonly use circulating I-FABP to evaluate gut damage as a measure of enterocyte cell lysis. However, in the absence of enterocyte lysis, I-FABP poorly correlates with microbial translocation [41]. Our results show that circulating I-FABP levels varied greatly throughout the course of a day, which limits its value as a marker of gut damage, since it is dependent on the time of sampling and fasting status. In contrast to I-FABP, REG3α appeared stable over the course of 24 h. Therefore, our results and previous work favor REG3α as a reliable gut damage marker independent of sampling time and food intake in PLWH [41].
LPS is a bacterial translocation marker, responsible for chronic immune activation in HIV-infected patients [51]. However, increasing evidence indicates that diet and food intake affect the plasma level of LPS in mouse and human models. Cani et al. [47] first reported in 2007 that plasma levels of LPS increased after feeding mice with a high-fat diet. Furthermore, López-Moreno et al. [52] reported that the consumption of diet rich in saturated fat increased plasma levels of LPS which in turn, increase the postprandial inflammatory response in subjects with metabolic syndrome. Our results also indicated that food intake was associated with an increase in plasma level of LPS in ART-treated PLWH up to 4 hours after lunch and supper. Although the underlying mechanism is unclear, it may be related to changes in microbiota composition, increases in the proportion of LPS producing Gramnegative bacteria in the presence of nutrients [53]. LPS detoxification by the intestinal alkaline phosphatase [54], or fat intake promoting gut translocation of LPS [47,52]. Therefore, monitoring LPS levels in PLWH should   c, f, i, l). Friedman test take into account feeding state and time of specimen acquisition. Unlike LPS, we showed that the fungal translocation marker BDG is stable throughout the day and independent of food intake. BDG can be found in food such as mushroom and seaweed [55,56]. Interestingly, Hashimoto et al. reported that serum BDG value was elevated due to intake of seaweed in a hematopoietic stem cell transplant recipient [57]. However, the elevation of BDG may have been linked to gut damage with increased intestinal permeability during acute graft-versus-host disease (GVHD). Nevertheless, our results showed that BDG is a reliable marker for fungal translocation in ART-treated PLWH. The food provided in our study did not comprise mushroom, seaweed or other material rich of BDG. Thus, further studies need be conducted in order to study the effects of BDG rich food on its plasma level.
We acknowledge that our study presents some limitations as we did not study the underlying mechanism of daily variation of I-FABP or LPS levels. Daily changes in the external environment may also influence those markers and studies have identified the molecular underpinnings of oscillations in circadian clock gene expression occurring over the 24-hour day [49]. Our study population only included a small sample size of male participants over the age of 50, therefore younger participants and inclusion of female participants will be needed to generalize study findings to a larger population, compared to a HIV-uninfected control group.

Conclusion
To our knowledge, we are the first to report the daily variation of different microbial translocation with gut damage markers in ART-treated PLWH. We showed that conversely to I-FABP and LPS, plasma levels of REG3α and BDG can be considered as reliable markers of gut damage and fungal translocation respectively, and are not influenced by food intake, time of sampling, or day/night shifts. Our findings provide reference for clinical research, focusing on the assessment of blood markers of gut damage and microbial translocation. The clinical implications of the daily variation of these markers should be assessed in larger cohorts of ART-treated PLWH, including male and female participants from different ages and ethnicity.