Table 3 Sequencing primers for complete HIV-1 PR, RT and IN sequencing
Primer (Direction) | Primer sequence | HXB2 | Gene |
|---|---|---|---|
RTC1F (Forward) | ACCTACACCTGTCAACATAATTG | 2486–2508 | PR and RT |
RTC2R (Reverse) | TGTCAATGGCCATTGTTTAACCTTTGG | 2630–2604 | PR and RT |
RTC3F (Forward) | CACCAGGGATTAGATATCAATATAATGTGC | 2965–2994 | PR and RT |
RTC4R (Reverse) | CTAAATCAGATCCTACATACAAGTCATCC | 3101–3129 | PR and RT |
KVL076 (Forward) | GCACAYAAAGGRATTGGAGGAAATGAAC | 4161–4188 | IN |
KVL082 (Forward) | GGVATTCCCTACAATCCCCAAAG | 4647–4669 | IN |
KVL083 (Reverse) | GAATACTGCCATTTGTACTGCTG | 4750–4772 | IN |
PAN2R (Reverse) | CTGCCATCTGTTTTCCATAYTC | 5037–5058 | IN |
Optional primers | |||
 2586F (Forward) | AAGCCAGGAATGGATGGCCCA | 2586–2606 | PR and RT |
 2713R (Reverse) | GGATTTTCAGGCCCAATTTTTG | 2713–2692 | PR and RT |
 PAN3F (Forward) | TTAAAAGAAAAGGGGGGATTGGG | 4783–4805 | IN |
 KVL084 (Reverse) | TCCTGTATGCARACCCCAATATG | 5243–5265 | IN |