Affordable drug resistance genotyping of HIV-1 reverse transcriptase, protease and integrase genes, for resource limited settings

Table 1 Reverse transcription and first-round PCR conditions

Reagent	Volume per reaction (µl)	Concentration per reaction
2X Reaction RT-PCR master mix	12.5	1X
Nuclease-free water	2.25	–
PANA2AF (5 µM)	2.5	0.5 µM
PANA3AR (5 µM)	2.5	0.5 µM
SSIV/platinum SuperFi DNA polymerase (2X)	0.25	0.02X
Total volume	20	–

cDNA complimentary DNA; RT-PCR reverse transcription polymerase chain reaction; SSIV SuperScript IV enzyme; µl microliter; µM micromolar; ^oC Degrees Celsius

ISSN: 1742-6405