Table 2 CRISPR/Cas systems for targeting proviral HIV-1 (In-vivo studies)
CRISPR/Cas system | Organism | Target region | Delivery | Results | References |
|---|---|---|---|---|---|
SaCas9 | Tg26 mice | LTR and viral genes | AAV9 (Adeno-associated vector) | Deletion at the target sequences in all the tissue samples studied. Excision of target sequence, reduction of 80–90% gag and env RNA | Kaminski et al. [48] |
SaCas9 | Tg26 mice NCr nude mouse BLT mice | LTR and viral genes | AAV (Adeno-associated vector) | Deletions at target sequences in samples collected from spleen, liver and bone-marrow. Excision and reduction of HIV gene expression | Yin et al. [59] |
SpCas9 | NRG rats | LTR and gag | Lentivirus | Cleavage and excision of integrated provirus in between the target sites | Bella et al. [60] |
SaCas9 | Humanized Mice (Engrafted with human CD34 + HSC) | LTR and gag | LASER ART therapy  +  AAV9 (Adeno-associated vector) | Effective decrease in viral load in dual treated mice compared to mice that were singularly treated with LASER ART or CRISPR alone In two of the seven mice, the viral load was undetectable | Dash et al. [62] |
SaCas9 | Rhesus macaques | LTR and gag | AAV9 (Adeno-associated vector) | Significant reduction of viral DNA in the blood and tissues. Precise cleavage and excision in samples collected from infected lymph nodes, spleen, bone-marrow and brain | Mancuso et al. [61] |