Table 1 Biochemical properties of ribonucleases and their zymogens
From: Ribonuclease zymogen induces cytotoxicity upon HIV-1 infection
Ribonuclease or peptide | kcat/KM (inactive) (M−1 s−1) | kcat/KM (activated) (M−1 s−1) | kcat/KM (relative) | Tm (°C) | kcat/KM (HIV-1 protease) (M−1 s−1) | Ki by RI (M) |
|---|---|---|---|---|---|---|
RNase 1 [21] | NA | 2.1 × 107 | NA | 57 | NA | 2.9 × 10−16 |
QBI-139 | NA | 4.3 × 105 | NA | 78 ± 2 | NA | 6 ± 1 × 10−10 |
SGIFLETS [20] | NA | NA | NA | NA | 5.0 × 105 | NA |
Str2a [16] | 5.8 × 103 | 6.7 × 107 | 11,000 | 47.5 | 3.9 × 103 | ND |
Str2-EVa | 2.0 × 102 | 8.5 × 103 | 42 | 50.0 ± 0.1 | 4.1 × 103 | 7 ± 1 × 10−10 |