Table 1 Drug resistance mutation profiles for accuracy of NGS against Sanger Sequencing
From: Next generation sequencing based in-house HIV genotyping method: validation report
Sample ID | Sequencing platform | RT region | PR region | ||
|---|---|---|---|---|---|
NRTI mutations | NNRTI mutations | Major mutations | Accessory | ||
DR-968–19 | Sanger Sequencing | K70R, M184V, K219Q | K103N, H221Y, M230L, L234I | None | None |
NGS | K70R, M184V, K219Q, D67G | K103N, H221Y, M230L, L234I | None | None | |
DR-969–19 | Sanger Sequencing | None | K103KNRS | None | None |
NGS | None | K103KNRS | None | None | |
DR-970–19 | Sanger Sequencing | M41L, D67N, M184V, T215F | A98G, G190A | N88S | V32A, L33F |
NGS | M41L, D67N, M184V, T215FIS | A98G, G190A | N88S | V32A, L33F | |
DR-971–19 | Sanger Sequencing | M41L, K65R, M184V, K219N | L100I, K103N | None | None |
NGS | M41L, K65R, M184V, K219N, T215FIS | L100I, K103N, G190A, A98G | N88S | V32A, L33F | |
DR-972–19 | Sanger Sequencing | D67N | K103N, K238T, Y188N | None | None |
NGS | D67GNS | K103N, K238T | None | None | |
DR-975–19 | Sanger Sequencing | M41L, M184V, L210W, T215F | A98G | M46I, I50V, I54V, V82A | L10F, L33F |
NGS | M41L, M184V, L210W, T215F | A98G, V108I | M46I, I50V, I54V, V82A | L10F, L33F | |
DR-976–19 | Sanger Sequencing | M184V | K101E, G190A, E138A | None | None |
NGS | K70R, M184V, K219Q | K101E, G190A, K103N, H221Y, M230L, L234I | None | None | |
DR-979–19 | Sanger Sequencing | T69DN, K70R, M184V | G190S | None | None |
NGS | T69DN, K70R, M184V | G190S | None | G48R | |
DR-980–19 | Sanger Sequencing | M184V, T215Y | K101E, E138A, G190A | None | None |
NGS | M184V, T215NSY | K101E, E138A, G190A, H221Y, P225H | M46I, L76V, I84V | Q58E | |
DR-981–19 | Sanger Sequencing | E44D | None | None | None |
NGS | E44D, K65E, M184IV | A98G, K103N, P225H | I54V, V82A | None |