Table 1 Nucleotide sequences of the primers used for real-time PCR
Target gene | Accession number | Type | Primer sequence | Productamplicon size (bp) |
|---|---|---|---|---|
IFN lambda 1 | NM_172140 | F R | GTGCTGGTGACTTTGGTGCTA GAGAAGCCTCAGGTCCCAAT | 225 |
IFN alpha R2 | NM_207584.2 | F R | GTCAGGCCTCTGCCACCTCT GGGTCCTGGAGTGGACTCTTTCT | 172 |
IFN gamma R1 | NM_000416.2 | F R | GCCAGGGTTGGACAAAAAGA GACTTCCTGCTCGTCTCCATTTA | 168 |
IFN lambda R1 | NM_170743.3 | F R | CCTCAGTGCCAGAACCATCTACA CCATGAGGGTCTTCCAGATCACA | 152 |
STAT1 | NM_007315.3 | F R | CCCTTCTGGCTTTGGATTGAA CTTCCCGGGAGCTCTCACTGA | 172 |
STAT2 | NM_198332.1 | F R | GAGAGCCCTCCTGGCAAGTTA GACGATTCACTGAAGCGCAGT | 185 |
STAT3 | NM_003150.3 | F R | TTGCCAGTTGTGGTGATCTCC GCTGCTCGATGCTCAGTCCTC | 199 |
STAT4 | NM_003151.3 | F R | TCAGGAAGGGCAAGCACTGGT GGAAGGGATGGGTAGCCAGGA | 150 |
STAT5A | NM_003152.3 | F R | GGATCCTGGTTGACGCCATGT GCTTGGATCCTCAGGCTCTCC | 122 |
STAT5B | NM_012448.3 | F R | GCTGGAAGCCTTGCTGATGCC CTGAGCTTGGATCCTCAGGCTCT | 114 |
STAT6 | NM_003153.4 | F R | GCCCACTCACTCCAGAGGACCT GGTGTTGGGGAAAGTCGACAT | 121 |
IRF9 | NM_006084.4 | F R | GAGCAAGTGGAGAGTGGGCAGTT CCAGACAGCTGGACCTCCTGTGT | 189 |
OAS1 | NM_016816.2 | F R | CAGTTAAATCGCCGGGGAGAGT GCTGGAGCGAACTCAGTACGAA | 101 |
MX1 | NM_002462.4 | F R | GGTGGTGGTCCCCAGTAATG CCGGCACTTGACAATCATGT | 150 |
Beta actin | NM_001101.3 | F R | GCACGGCATCGTCACCAACT GCTGGGGTGTTGAAGGTCTCA | 177 |