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Table 1 Examples of unintegrated DNAs in virus-infected cells and their fates

From: Distribution and fate of HIV-1 unintegrated DNA species: a comprehensive update

Virus Cell line Cell number hpi (hours per infection) Methods Consequence Ref.
Avian sarcoma virus QT-6   5 hpi   Closed circular DNA [34]
MLV NIH 3T3, TE671, ARPE-19 1 × 106 cells 2 hpi qPCR 2-LTR [16]
HIV-1 MT-2 5 × 105 cells 2 hpi PCR and Southern hybridization 1-LTR [31]
12 hpi 2-LTR
Monocyte 1 × 106 cells 2 hpi PCR and Southern hybridization 1-LTR
12 hpi 2-LTR
Macrophage 3 × 105 cells 4 dpi qPCR 2-LTR 30–35 copies/100cells [45]
21 dpi   6 copies/100cells
CD4+ T lymphocytes 3 × 105 cells 4 dpi 2-LTR 11 copies/100cells
14 dpi X
Jurkat 1 × 107 3 dpt 2-LTR 8.97% [36]
1-LTR 46.21%
293T   2-LTR 5.42%
1-LTR 40.16%
  SupT1 (lymphoid cells) 5 × 106 cells 15 hpi 2-LTR 0.01 copy/cell [46]
12 hpi 40 copies/cells
  1. dpt days post-transduction, dpi days post infection