Fig. 9

The fitness difference of AHI HIV-1env chimeric virus relative to the control HIV-1env chimeric virus derived from chronic infection. As described in the schematic (a), HIV-1env_B chimeric viruses were competed against three reference viruses (i.e. HIVenv_I10, HIVenv_K44, and HIVenv_Q0) in HIV-negative PBMCs as described in the “Methods” section. a is also schematic describing the PCR and 454 pyrosequencing methods used to detect and quantify dual infection (see “Methods” section for more detail). The percent replication relative to HIVenv_I10, HIVenv_K44, and HIVenv_Q0 is shown in b. Total relative fitness (the addition of all relative fitness values) is presented in c to provide a rank order for relative fitness for the 19 HIVenv viruses tested