Fig. 1
Production of pREC_nfl_SHIVenv containing the gp120 coding region derived from acute HIV-1 infections. a Construction of pREC_nfl_SHIVKB9_envB vector/URA3. The URA3 gene and the corresponding AHI env B genes only replace a region flanked by KB9 sequence (HXB2 numbering nt5996-nt8638). SHIVKB9 is a SIVmac239 virus containing only a portion of the HIV-1 env89.6 gene, i.e. from the 1st exon of tat to the end on the 2nd exon of tat (HXB2 numbering nt5823-nt8676, shadowed in yellow). b 20 acute HIV-1 subtype B envelope (Env) and three chronic HIV-1 Envs (i.e. I10, K44, and Q0) were cloned into pREC_nfl_SHIVKB9Δenv/URA3 (or pREC_nfl_HIVΔenv/URA3) by homologous yeast recombination/gap repair to produce the pREC_nfl_SHIVKB9_envB1-20 and pREC_nfl_HIVenv_B1-20 vectors