Fig. 2
From: Defining the fitness of HIV-1 isolates with dual/mixed co-receptor usage
Neighbor joining phylogenic trees of two dual tropic HIV-1 isolates, two HIV-1 env chimeric viruses, and of the sampled clones in their virus population. Alignments and phylogenetic trees were constructed for the C2-V3 region (350 nt) of the four reference strains, r5 B2, r5 C3, x4 A8, and x4 E6, the two dual tropic HIV-1 isolates (C19 and C27), the two HIV-1 env chimeric viruses (B12 and B19) and set of reference strains (a). As described in the “Methods”, the Env coding region of two dual/mixed (dm) HIV-1 isolates were PCR amplified and cloned into pREC env via yeast-based recombination. The C2-V3 sequences from 21 B19 (b), 18 B19 (c), 26 C19 (d), 21 © clones (e) were aligned using MUSCLE and then schematic represented in phylogenetic trees.