Titrations of a select series of related combinations with individual reporters. We measured the titrated activities for a single series of related combinations (2.2, 4.2 and 6.3) and their corresponding single shRNA plasmids (#3, #8, #9, #2, #7, and #6) using shRNA-specific reporters. We overlaid connection lines between the maximum dose values for each shRNA/reporter to show the positional relationship of each shRNA in the combinations for comparison to the equivalent measurements using the aio reporters. We expanded the titration points for the same series of plasmids tested with the aio sense reporter, and included them on the same scale for comparison. n.b. we were unable to test titrated amounts of shRNA #2 with the Gag-500 reporter due to stock contamination. Values shown incorporate representative data from 2 independent experiments.