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Figure 3 | AIDS Research and Therapy

Figure 3

From: Inhibition of human immunodeficiency virus type-1 by cdk inhibitors

Figure 3

Alsterpaullone inhibition of cdk2 and cyclin expression in HIV-1 infected and uninfected cells. A) HIV-1 infected OM10.1 and uninfected Jurkat cells were treated with alsterpaullone (0.01, 0.1, 0.5, and 1 μM) for 48 hours. Total cell extracts (25 μg) were subjected to western blot analysis for cdk2, cyclin A, cyclin E, cyclin T, GSK-3α, GSK-3β, and actin. B) Similar to panel A, Jurkat and OM10.1 cells were treated with various concentrations of alsterpaullone and cell extracts were processed for presence of apoptosis markers (cleaved PARP and caspase-3 products). β-actin was used as internal control for all westerns. Both processed and cleaved PARP and caspase-3 were observed in higher concentrations of alsterpaullone treated OM10.1 infected cells.

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