Figure 3

Differential effects of H69 mutations on protease autoprocessing. pEBG-derived plasmids expressing the indicated fusion proteins were transfected into HEK293T cells using calcium phosphate and post-nuclear cell lysates were prepared at 40 h post transfection. Aliquots (~20 μL) of each sample were subjected to western blotting in parallel using monoclonal mouse anti-Flag or anti-HA primary antibodies and IR800 goat anti-mouse second antibody. The full-length fusion precursor and processing products are indicated in the middle; molecular mass markers (kDa) are indicated at left.