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Figure 2 | AIDS Research and Therapy

Figure 2

From: Alkylating HIV-1 Nef - a potential way of HIV intervention

Figure 2

TPCK-modification of Nef mutants with Cys55, Cys142, and/or Cys206 substituted with Ala. Plasmid encoding Nef mutant C55/A, C206/A, C142&206/A, C55&206/A, C55&142/A or C55&C142&c206/A (Cys free) were transfected into JTAg cells. TPCK modification was determined as described in Fig. 1. Arrows indicate the Cys206-modified (C206M), Cys55-modified (C55M) and the un-modified Nef (un) proteins.

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