Two-dimensional gel electrophoresis of pooled patient samples. Post sample depletion, six 2D gels (IPG Strip pH 3-10, 4-20% SDS-PAGE) were run in tandem to separate the low and high abundance protein fractions of pooled patient serum samples in tandem. Gels a, b, and c are representative of LTNP, HAART, and Negative low abundance patient samples respectively. Gels d, e, and f are representative of LTNP, HAART, and negative high abundance patient samples respectively. Arrows and circles indicate protein spots excised for MALDI-TOF analysis.