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Table 3 Inhibition of the production of HIV-1BaL or HIV-1NDK in immature monocyte-derived dendritic cells by microbicide molecule candidates

From: Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization

  HIV-1BaL HIV-1NDK
  NonOps HI NonOps Ops NonOps HI NonOps Ops
Lf I (30%)* I (26%) NI [S]** I (63%) I (61%) I (26%) [S]
CADA I (61%) I (64%) NI [S] I (95%) I (93%) I (96%)
T20 I (83%) I (82%) I (57%) [S] I (95%) I (95%) I (97%)
IgG1B12 I (86%) I (85%) I (60%) [S] I (94%) I (96%) I (94%)
IgG12G5 NI NI NI I (95%) I (95%) I (54%) [S]
IgG2G12 I (67%) I (62%) I (69%) I (96%) I (95%) I (96%)
IgG2F5 I (84%) I (77%) I (41%) [S] I (97%) I (96%) I (97%)
Ab to gp160*** I (61%) I (58%) I (50%) [S] I (79%) I (91%) I (69%)
  1. * Percentage of virus production inhibition in brackets
  2. ** Significant difference between the percentages of production inhibition is significant according to Ops, HI NonOps and NonOps HIV-1 (Mann & Whitney U test)
  3. *** Used as positive control
  4. NonOps: Non opsonized free HIV-1; HI NonOps: Heat inactivated non opsonized free HIV-1; Ops: Free HIV-1 opsonized virus by complement components
  5. I: Virus production inhibition; NI: Lack of transcytosis inhibition
  6. S: Significant
  7. The infection inhibition is shown for the best doses of the candidate molecules, and is expressed as percentage of the average of three independent experiments. The range of detected HIV-1 p24 antigen for uninhibited HIV-1 replication in negative control experimentations (without microbicide molecules) was 800-1000 pg/ml in iMDDC infectivity assay. The capability of dendritic cells to replicate HIV-1 is donor-dependent.