Figure 2

Protection of cells from HIV infection after removal of inhibitor from culture. The protection of cells from HIV infection was determined using a TZM-bl indicator cell line based-single cycle replication assay described in the "Materials and Methods" section and illustrated in Figure 1A. TZM-bl cells were cultured in the presence of various concentrations inhibitor (LFD4C, FTC, DDI, or 3TC) 24 h. The cells were then washed to remove extracellular drug, and infected with HIV-1 IIIB virus at an MOI of 0.1 at 0, 24, and 48 h of drug removal. The percentage inhibition of HIV-1 replication was determined by measuring the luciferase activity. The curves represent: percent inhibition of HIV-1 replication when cells were infected at the time of drug treatment and incubated together for 24 h (black circle); percent inhibition when cells were pre-incubated with drugs for 24 h and the media changed without replacement of drug prior to infection and incubated for 24 h (black triangle); and percent inhibition when cells were pre-incubated with drugs for 24 h and then the media was changed to remove drug at 0 and 24 h without drug replacement prior to infection (black square). Results are the average of at least three independent experiments. Error bars indicate standard deviations.