Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo

Table 1 Inhibition of HIV-1 replication in human TCBs by WR1065 and amifostine.

Concentration (μM)	Number of experiments	% Inhibition of^a HIV-1 replication (mean ± SE)	% Viability^b no HIV-1 Infection (Trypan blue)	% Viability^c HIV-1 Infection (Annexin V)
*WR1065*
26	5	65.0 ± 7.5	77.8 ± 4.2	95.8 ± 0.5
52	7	88.7 ± 5.5	49.3 ± 3.8	92.5 ± 1.1
103	8	93.9 ± 3.1	36.8 ± 17.1	ND^d
*Amifostine*
50	4	75.4 ± 8.3	53.2 ± 9.6	ND^d

^a Human TCBs were infected with HIV-1 for 2 hr before incubation for 72 hr with WR1065, or amifostine converted to WR-1965 by pre-incubation with alkaline phosphatase. 50% inhibition of virus replication was at 13 μM WR1065.
^b Cell viability (mean ± SE), in HIV-1-uninfected cells, as determined by Trypan blue exclusion.
^c Cell viability (mean ± range, n = 2 experiments), in HIV-1-infected cells, was determined by Annexin V.
^dND = not determined.

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