Table 2 The primers used for nested PCR/RT nested PCR amplification

ED12

AGT GCT TCC TGC TGC TCC CAA GAA CCC AAG

RT primer for HIV env gp120

ED31

CCT CAG CCA TTA CAC AGG CCT GTC CAA AG

1^st round PCR forward primer for HIV env gp120

BH2

CCT TGG TGG GTG CTA CTC CTA ATG GTT CA

1^st round PCR reverse primer for HIV env gp120

DR7

TCA ACT CAA CTG CTG TTA AAT GGC AGT CTA GC

2^nd round PCR forward primer for HIV env gp120

DR8

CAC TTC TCC AAT TGT CCC TCA TAT CTC CTC C

2^nd round PCR reverse primer for HIV env gp120

Hu-CD4 RT primer

ATG TCT TCT GAA ACC GGT GAG GAC ACT G

RT primer for human CD4 mRNA

Hu-CD4 outside F

CCA AGT CTT GGA TCA CCT TTG ACC TGA AG

1^st round PCR forward primer for Human CD4 cDNA

Hu-CD4 outside R

AGA AGA AGA TGC CTA GCC CAA TGA AAA GC

1^st round PCR reverse primer for Human CD4 cDNA

Hu-CD4 Inside F

CTC CCG CTC CAC CTC ACC CTG

2^nd round PCR forward primer for Human CD4 cDNA

Hu-CD4 Inside R

CAT GTG GGC AGA ACC TTG ATG TTG G

2^nd round PCR reverse primer for Human CD4 cDNA

B-globin outside F

CTG CTG GTG GTC TAC CCT TGG AC

1^st round PCR primer for Human Beta globin DNA

B-globin outside R

CTC AAG TTC TCA GGA TCC A

1^st round PCR primer for Human Beta globin DNA

B-globin inside F

GGT TCT TTG AGT CCT TTG GGG ATC

2^nd round PCR forward primer for Human Beta globin DNA

B-globin inside R

GTC ACA GTG CAG CTC ACT CAG TGT G

2^nd round PCR reverse primer for Human Beta globin DNA

B-actin outside F

GCA CCA CAC CTT CTA CAA TG

1^st round PCR primer for Human Beta actin cDNA

B-actin outside R

TGC TTG CTG ATC CAC ATC TG

1^st round PCR primer for Human Beta actin cDNA

B-actin inside F

TAC CAC TGG CAT CGT GAT GGA CTC

2^nd round PCR primer for Human Beta actin cDNA

B-actin inside R

CGC TCA TTG CCA ATG GTG ATG AC

2^nd round PCR primer for Human Beta actin cDNA

Gag outside F

GGC CAT ATC ACC TAG AAC TTT AAA TGC ATG G

1^st round PCR primer for HIV Gag

Gag outside R

CCT ACT GGG ATA GGT GGA TTA TTT GTC ATC CA

1^st round PCR primer for HIV Gag

Gag inside F

GGC ACA TCA AGC AGC CAT GCA AAT G

2^nd round PCR primer for HIV Gag

Gag inside R

TAG TTC CTG CTA TGT CAC TTC CCC TTG G

2^nd round PCR primer for HIV Gag