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Table 2 The primers used for nested PCR/RT nested PCR amplification

From: Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy

Name Sequences (5'→3') Description
ED12 AGT GCT TCC TGC TGC TCC CAA GAA CCC AAG RT primer for HIV env gp120
ED31 CCT CAG CCA TTA CAC AGG CCT GTC CAA AG 1st round PCR forward primer for HIV env gp120
BH2 CCT TGG TGG GTG CTA CTC CTA ATG GTT CA 1st round PCR reverse primer for HIV env gp120
DR7 TCA ACT CAA CTG CTG TTA AAT GGC AGT CTA GC 2nd round PCR forward primer for HIV env gp120
DR8 CAC TTC TCC AAT TGT CCC TCA TAT CTC CTC C 2nd round PCR reverse primer for HIV env gp120
Hu-CD4 RT primer ATG TCT TCT GAA ACC GGT GAG GAC ACT G RT primer for human CD4 mRNA
Hu-CD4 outside F CCA AGT CTT GGA TCA CCT TTG ACC TGA AG 1st round PCR forward primer for Human CD4 cDNA
Hu-CD4 outside R AGA AGA AGA TGC CTA GCC CAA TGA AAA GC 1st round PCR reverse primer for Human CD4 cDNA
Hu-CD4 Inside F CTC CCG CTC CAC CTC ACC CTG 2nd round PCR forward primer for Human CD4 cDNA
Hu-CD4 Inside R CAT GTG GGC AGA ACC TTG ATG TTG G 2nd round PCR reverse primer for Human CD4 cDNA
B-globin outside F CTG CTG GTG GTC TAC CCT TGG AC 1st round PCR primer for Human Beta globin DNA
B-globin outside R CTC AAG TTC TCA GGA TCC A 1st round PCR primer for Human Beta globin DNA
B-globin inside F GGT TCT TTG AGT CCT TTG GGG ATC 2nd round PCR forward primer for Human Beta globin DNA
B-globin inside R GTC ACA GTG CAG CTC ACT CAG TGT G 2nd round PCR reverse primer for Human Beta globin DNA
B-actin outside F GCA CCA CAC CTT CTA CAA TG 1st round PCR primer for Human Beta actin cDNA
B-actin outside R TGC TTG CTG ATC CAC ATC TG 1st round PCR primer for Human Beta actin cDNA
B-actin inside F TAC CAC TGG CAT CGT GAT GGA CTC 2nd round PCR primer for Human Beta actin cDNA
B-actin inside R CGC TCA TTG CCA ATG GTG ATG AC 2nd round PCR primer for Human Beta actin cDNA
Gag outside F GGC CAT ATC ACC TAG AAC TTT AAA TGC ATG G 1st round PCR primer for HIV Gag
Gag outside R CCT ACT GGG ATA GGT GGA TTA TTT GTC ATC CA 1st round PCR primer for HIV Gag
Gag inside F GGC ACA TCA AGC AGC CAT GCA AAT G 2nd round PCR primer for HIV Gag
Gag inside R TAG TTC CTG CTA TGT CAC TTC CCC TTG G 2nd round PCR primer for HIV Gag