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Figure 3 | AIDS Research and Therapy

Figure 3

From: Comparison of metal-dependent catalysis by HIV-1 and ASV integrase proteins using a new and rapid, moderate throughput assay for joining activity in solution

Figure 3

Joining activity confirmed with gel electrophoresis. Left, sequences of the donor oligodeoxynucleotides used in the joining assay. The location of carboxyfluorescein (FAM), 5' radioactive 32P, and 3' biotin are shown. The -A substrate removes only the A of the conserved CA dinucleotide while the -CA substrate removes both residues. Right, lanes 1 through 3 show HIV-1 IN joining activity on its substrate after 0, 60, 120 min of incubation, respectively. Lanes 4 through 6, 7 through 9, and 10 through 12, show ASV IN joining activity after 0, 15, 30 min of incubation.

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