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Figure 2 | AIDS Research and Therapy

Figure 2

From: The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT

Figure 2

Magnitude of IFN-γ+ MIP-1β+ T-cells and IFN-γ+ T-cells in mock stimulated samples. Percentages of total IFN-γ+ and IFN-γ+ MIP-1β+ CD8 (A) or CD4 (B) T-cells are shown. The lines indicate the median percentage of the observed background. P values were determined by Wilcoxon matched pairs test. In (C) representative data from one study subject are shown. PBMC are gated on CD8+ CD3+ lymphocytes and were stimulated as indicated at the top of the figure. The peptide LDLWIYHTQGYFPDWQNY (LY18), included in pool 8, was here used alone. Data were analyzed with the IFN-γ+ MIP-1β+ (upper row) or the total IFN-γ+ (bottom row) data analysis system. The percentage of IFN-γ+ MIP-1β+ and total IFN-γ+ CD8 T-cells is indicated in the upper-right corner of each plot. Samples were scored as positive or negative (upper-left corner) according to the following procedure. After background subtraction, the 90 percentile of the negative values was calculated and this value was considered as a threshold. Samples were considered positive when higher than the threshold and at least 2 times higher than their respective mock stimulated control.

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