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Figure 3 | AIDS Research and Therapy

Figure 3

From: Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance

Figure 3

HIV-1 challenge of siRNA transposed GHOST-R3/X4/R5 cells. To determine viral resistance, siRNA transposed transgenic cells were challenged with HIV-1 NL4.3 (CXCR4 tropic virus), HIV-1 BaL (CCR5 tropic virus) or HIV-1 89.6 (dual tropic virus) viruses at an MOI of 0.01. On various days post-infection, cell culture supernatants were collected and analyzed for p24 antigen levels by ELISA to determine the levels of viral inhibition. Untransposed (), control RFP transposed (■), CXCR4 siRNA transposed (×) or CCR5 siRNA transposed (). Panel A – NL4.3, Panel B – BaL, Panel C – 89.6.

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