Figure 6

Double drugs treatment results in lower p19 Gag levels in HTLV-1 infected cells. A) MT-2 cells (HTLV-1 infected) were treated with TNF-α (10 ng/ml) for 2 h, washed, and subsequently treated with a specific NF-kB or CDK inhibitor. Activated cells were subsequently treated them with BMS-345541 alone (0.1 μM), Purvalanol A alone (0.5 μM), or a combination of both drugs. Samples were collected after 7 days and used for detection of p19 Gag using ELISA. B) Log phase 293 cells were transfected with ACH.pcTax (wild type HTLV-1 clone, generous gift of Dr. Lee Ratner, Washington University) using electroporation method. After transfection, the cells were cultured in complete medium and culture supernatants were collected at 4 days post-transfection, and virus particle production was monitored by p19 ELISA. Drug treatments (as in panle A) were 6 hrs after transfection of the 293 cells for a total of 150 hrs.