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Table 1 Efficiency of production of Rev M10 encoding vector stocks using various combinations of packaging and gene transfer vectors containing RRE from HIV-1 or SIVmac239

From: Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes

Packaging Plasmid/RRE Source

Gene Transfer Vector/RRE Source

Vector amount used

Rev Source

SEAP-adjusted Titer (IU/ml) (mean ± SD)

Fold difference in Titera

Mock

   

0

 

pGP/HIV-1

pN-EF1α-EGFP/HIV-1

3.00 μg

HIV-1

2.0 ± 0.2 × 105

1

 

pN-EF1α-EGFP-2A-M10/HIV-1

0.75 μg

HIV-1

7.8 ± 3.1 × 102

254

 

pN-EF1α-EGFP-2A-M10/HIV-1

1.50 μg

HIV-1

3.7 ± 0.6 × 102

537

 

pN-EF1α-EGFP-2A-M10/HIV-1

3.00 μg

HIV-1

2.3 ± 1.0 × 102

862

pGP/HIV-1

pN-EF1α-EGFP/SIV

3.00 μg

HIV-1

5.1 ± 0.2 × 105

1

 

pN-EF1α-EGFP-2A-M10/SIV

0.75 μg

HIV-1

5.5 ± 2.3 × 103

93

 

pN-EF1α-EGFP-2A-M10/SIV

1.50 μg

HIV-1

1.0 ± 0.1 × 104

50

 

pN-EF1α-EGFP-2A-M10/SIV

3.00 μg

HIV-1

1.1 ± 0.1 × 104

47

pGP/SIVb

pN-EF1α-EGFP/HIV-1

3.00 μg

HIV-1

1.1 ± 0.1 × 105

1

 

pN-EF1α-EGFP-2A-M10/HIV-1

0.75 μg

HIV-1

9.5 ± 0.3 × 102

119

 

pN-EF1α-EGFP-2A-M10/HIV-1

1.50 μg

HIV-1

5.8 ± 2.3 × 102

196

 

pN-EF1α-EGFP-2A-M10/HIV-1

3.00 μg

HIV-1

5.7 ± 0.1 × 102

201

pGP/SIV

pN-EF1α-EGFP/SIV

3.00 μg

HIV-1

1.8 ± 0.0 × 105

1

 

pN-EF1α-EGFP-2A-M10/SIV

0.75 μg

HIV-1

2.7 ± 0.3 × 104

7

 

pN-EF1α-EGFP-2A-M10/SIV

1.50 μg

HIV-1

3.2 ± 0.3 × 104

6

 

pN-EF1α-EGFP-2A-M10/SIV

3.00 μg

HIV-1

3.0 ± 0.0 × 104

6

pGP/SIV

pN-EF1α-EGFP/SIV

3.00 μg

SIVb

9.6 ± 1.1 × 104

1

 

pN-EF1α-EGFP-2A-M10/SIV

0.75 μg

SIV

4.7 ± 0.1 × 103

20

 

pN-EF1α-EGFP-2A-M10/SIV

1.50 μg

SIV

5.1 ± 0.1 × 103

19

 

pN-EF1α-EGFP-2A-M10/SIV

3.00 μg

SIV

5.8 ± 0.3 × 103

17

  1. a Fold difference in titer was determined by dividing the titer of the control vector encoding EGFP alone by the titer of the corresponding vector encoding EGFP and Rev M10.
  2. bSIV refers to the molecular clone SIVmac239.