Packaging Plasmid/RRE Source | Gene Transfer Vector/RRE Source | Vector amount used | Rev Source | SEAP-adjusted Titer (IU/ml) (mean ± SD) | Fold difference in Titera |
---|---|---|---|---|---|
Mock | Â | Â | Â | 0 | Â |
pGP/HIV-1 | pN-EF1α-EGFP/HIV-1 | 3.00 μg | HIV-1 | 2.0 ± 0.2 × 105 | 1 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 0.75 μg | HIV-1 | 7.8 ± 3.1 × 102 | 254 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 1.50 μg | HIV-1 | 3.7 ± 0.6 × 102 | 537 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 3.00 μg | HIV-1 | 2.3 ± 1.0 × 102 | 862 |
pGP/HIV-1 | pN-EF1α-EGFP/SIV | 3.00 μg | HIV-1 | 5.1 ± 0.2 × 105 | 1 |
 | pN-EF1α-EGFP-2A-M10/SIV | 0.75 μg | HIV-1 | 5.5 ± 2.3 × 103 | 93 |
 | pN-EF1α-EGFP-2A-M10/SIV | 1.50 μg | HIV-1 | 1.0 ± 0.1 × 104 | 50 |
 | pN-EF1α-EGFP-2A-M10/SIV | 3.00 μg | HIV-1 | 1.1 ± 0.1 × 104 | 47 |
pGP/SIVb | pN-EF1α-EGFP/HIV-1 | 3.00 μg | HIV-1 | 1.1 ± 0.1 × 105 | 1 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 0.75 μg | HIV-1 | 9.5 ± 0.3 × 102 | 119 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 1.50 μg | HIV-1 | 5.8 ± 2.3 × 102 | 196 |
 | pN-EF1α-EGFP-2A-M10/HIV-1 | 3.00 μg | HIV-1 | 5.7 ± 0.1 × 102 | 201 |
pGP/SIV | pN-EF1α-EGFP/SIV | 3.00 μg | HIV-1 | 1.8 ± 0.0 × 105 | 1 |
 | pN-EF1α-EGFP-2A-M10/SIV | 0.75 μg | HIV-1 | 2.7 ± 0.3 × 104 | 7 |
 | pN-EF1α-EGFP-2A-M10/SIV | 1.50 μg | HIV-1 | 3.2 ± 0.3 × 104 | 6 |
 | pN-EF1α-EGFP-2A-M10/SIV | 3.00 μg | HIV-1 | 3.0 ± 0.0 × 104 | 6 |
pGP/SIV | pN-EF1α-EGFP/SIV | 3.00 μg | SIVb | 9.6 ± 1.1 × 104 | 1 |
 | pN-EF1α-EGFP-2A-M10/SIV | 0.75 μg | SIV | 4.7 ± 0.1 × 103 | 20 |
 | pN-EF1α-EGFP-2A-M10/SIV | 1.50 μg | SIV | 5.1 ± 0.1 × 103 | 19 |
 | pN-EF1α-EGFP-2A-M10/SIV | 3.00 μg | SIV | 5.8 ± 0.3 × 103 | 17 |