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Figure 3 | AIDS Research and Therapy

Figure 3

From: Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes

Figure 3

Determination of optimal amounts of pCI-HIV-1 Rev for production of vector stocks with the SIV RRE-based HIV-1 packaging system. Vector stocks were produced in 293T cells using pGP/SIV 1045 RRE and pN-EF1α-EGFP-WPRE/SIV RRE with indicated amounts of pCI-HIV Rev or pCI-SIV Rev. The transfections also included pCMVtat, pMD.G and a SEAP expression construct. The vector stocks were used for infection of 293T cells and the resultant SEAP-adjusted vector titers are shown. Error bar = 1 SD.

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AIDS Research and Therapy

ISSN: 1742-6405

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