Antigen uptake by hES-DCs: Cultured hES and FL DCs were sorted based on CD1a marker. The cells were then incubated with Alexa-Dextran at 0°C and 37°C for 1 hr and analyzed by FACS as described in Methods. The percent antigen uptake was measured as the difference in percentages between the test (37°C) and control (0°C). The percent positive cells are indicated in the plots for both hES-DCs and FL-DCs. Data are representative of triplicate experiments.