Figure 6

Deletion of aptamer cassette in RNA transcripts from the Phoenix(amphotropic) packaging cell lines. The Phoenix (amphotropic) packaging cell lines were transfected with the different aptamer vectors. Total RNA was isolated and subjected to amplification by RT-PCR with (+RT) or without (-RT) reverse transcriptase using the 3'GFP-for and 3' UTR-rev primers. Amplification of the MMP 70.15 plasmid was included as control. The DNA ladder is the 100 bp DNA ladder from Invitrogen.